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Oral rifampicin interferes with urine dipstick tests for patients with pulmonary tuberculosis

Fei Wang1, Lihui Zhao1, Wei Du1

  • 1Department of Laboratory Medicine, Wuhan Pulmonary Hospital, Wuhan, China.

Journal of Thoracic Disease
|June 16, 2023
PubMed
Abstract

Insights

Rifampicin and its metabolite interfere with urine dipstick tests in tuberculosis patients. Collecting urine 12-24 hours after rifampicin administration can prevent these interferences.

Area of Science:

  • Clinical Chemistry
  • Pharmacology
  • Microbiology

Background:

  • Rifampicin (RMP) and its metabolite desacetyl rifampicin (dRMP) are used to treat tuberculosis (TB).
  • These compounds can interfere with standard urine dipstick tests (UDTs).

Purpose of the Study:

  • To investigate the impact of RMP and dRMP on UDTs using two distinct dipstick brands.
  • To determine the concentration ranges of RMP and dRMP causing interference.

Main Methods:

  • Urine colorimetry was used to measure RMP concentrations at 2-6 and 12-24 hours post-administration.
  • In vitro interference assays and confirmatory tests were conducted on Arkray's Aution Sticks 10EA and GIMA's Combi-Screen 11SYS Plus.

Main Results:

  • Significant interference was observed for protein, leukocyte esterase, ketones, and nitrite analytes across both dipstick types.
  • Interference levels varied with RMP concentrations, ranging from 125 µg/mL to 500 µg/mL depending on the analyte and dipstick.

Conclusions:

  • RMP and dRMP interfere with UDTs to varying degrees.
  • In vitro assays are not fully reliable substitutes for confirmatory tests.
  • Sampling urine 12-24 hours after RMP intake minimizes interference, improving test accuracy.
Keywords:
Interferencerifampicin (RMP)urine dipstick test (UDT)

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