Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.1K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.1K
Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

2.6K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.6K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

8.7K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.7K
MicroRNAs01:22

MicroRNAs

3.0K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
3.0K
Pleiotropy01:33

Pleiotropy

40.7K
Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
40.7K
Abnormal Proliferation02:23

Abnormal Proliferation

4.6K
Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Expression and clinical diagnostic value of miR-383 in patients with severe preeclampsia.

Cellular and molecular biology (Noisy-le-Grand, France)·2020
Same author

FNDC5 Attenuates Oxidative Stress and NLRP3 Inflammasome Activation in Vascular Smooth Muscle Cells via Activating the AMPK-SIRT1 Signal Pathway.

Oxidative medicine and cellular longevity·2020
Same author

Obesity-induced excess of 17-hydroxyprogesterone promotes hyperglycemia through activation of glucocorticoid receptor.

The Journal of clinical investigation·2020
Same author

Human neutralizing antibodies elicited by SARS-CoV-2 infection.

Nature·2020
Same author

Extensive intracranial arterial dolichoectasia involving distal branches of intracranial arteries: two cases report and review of the literature.

The International journal of neuroscience·2020
Same author

Photocaged FRET nanoflares for intracellular microRNA imaging.

Chemical communications (Cambridge, England)·2020

Related Experiment Video

Updated: Jul 26, 2025

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

25.4K

The long non-coding RNA MALAT1 regulates intestine host-microbe interactions and polyposis.

Tianyun Long1, Juan E Hernandez1, Shengyun Ma1

  • 1Department of Cellular and Molecular Medicine, University of California San Diego, La Jolla, CA, United States.

Frontiers in Cell and Developmental Biology
|June 16, 2023
PubMed
Summary

The long non-coding RNA MALAT1 influences gut microbes and cancer. It restricts early cancer but promotes later progression, impacting patient survival.

Keywords:
MALAT1intestinal cancerintestine epithelial cellsintestine microbeslong non-coding RNA

More Related Videos

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos
07:24

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos

Published on: December 13, 2018

6.5K
Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity
07:09

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity

Published on: January 7, 2019

7.5K

Related Experiment Videos

Last Updated: Jul 26, 2025

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
09:36

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

Published on: April 10, 2018

25.4K
Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos
07:24

Repression of Multiple Myeloma Cell Growth In Vivo by Single-wall Carbon Nanotube SWCNT-delivered MALAT1 Antisense Oligos

Published on: December 13, 2018

6.5K
Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity
07:09

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity

Published on: January 7, 2019

7.5K

Area of Science:

  • Molecular Biology
  • Genomics
  • Microbiome Research

Background:

  • The long non-coding RNA (lncRNA) MALAT1 is known for maintaining intestinal epithelial barrier integrity and regulating inflammation.
  • Its role in modulating intestinal microbial communities and cancer development susceptibility is largely unknown.

Purpose of the Study:

  • To investigate the function of MALAT1 in regulating host anti-microbial responses and gut microbial composition.
  • To determine MALAT1's role in intestinal tumorigenesis and its impact on cancer progression.

Main Methods:

  • Utilized the APC mutant mouse model for intestinal tumorigenesis.
  • Performed gene expression analysis and genomic assays to identify MALAT1 targets and mechanisms.
  • Analyzed mucosal-associated microbial communities and polyp characteristics.

Main Results:

  • MALAT1 regulates anti-microbial gene expression and regional gut microbial composition.
  • Loss of MALAT1 increased polyp counts but reduced polyp size in the APC mouse model.
  • MALAT1 levels, specifically ZNF638 and SENP8, predict patient survival in colon adenoma.

Conclusions:

  • MALAT1 exhibits a dual role in cancer, restricting early stages while promoting later progression.
  • MALAT1 influences intestinal homeostasis, microbial communities, and cancer pathogenesis through direct and indirect mechanisms.