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Updated: Jun 25, 2026
Efficient Nucleic Acid Extraction and 16S rRNA Gene Sequencing for Bacterial Community Characterization
Published on: April 14, 2016
Sample Collection, DNA Extraction, and Library Construction Protocols of the Human Microbiome Studies in the
Yetong Wang1, Ruyi Zhang2, Yanni Pu1
1State Key Laboratory of Genetic Engineering, Human Phenome Institute, and School of Life Sciences, Fudan University, 2005 Songhu Road, Shanghai, 200433 China.
Abstract:
The human microbiome plays a crucial role in human health. In the past decade, advances in high-throughput sequencing technologies and analytical software have significantly improved our knowledge of the human microbiome. However, most studies concerning the human microbiome did not provide repeatable protocols to guide the sample collection, handling, and processing procedures, which impedes obtaining valid and timely microbial taxonomic and functional results. This protocol provides detailed operation methods of human microbial sample collection, DNA extraction, and library construction for both the amplicon sequencing-based measurements of the microbial samples from the human nasal cavity, oral cavity, and skin, as well as the shotgun metagenomic sequencing-based measurements of the human stool samples among adult participants. This study intends to develop practical procedure standards to improve the reproducibility of microbiota profiling of human samples.
Supplementary Information:
The online version contains supplementary material available at 10.1007/s43657-023-00097-y.
