Nucleic acid aptamer-based biosensors and their application in thrombin analysis

Area of Science:

• Biochemistry and Molecular Biology
• Biomedical Engineering
• Analytical Chemistry

Background:

• Thrombin is a crucial serine protease regulating blood coagulation and anticoagulation.
• Aptamers, short nucleic acid sequences, are increasingly utilized in biosensor development due to their specificity, cost-effectiveness, and biocompatibility.

Purpose of the Study:

• To review recent advancements in aptamer-based biosensors for thrombin quantification.
• To focus on optical and electrochemical sensing strategies for thrombin detection.

Main Methods:

• Review of literature on aptamer-based biosensors for thrombin.
• Analysis of optical sensor technologies (e.g., fluorescence, colorimetric).
• Analysis of electrochemical sensor technologies (e.g., voltammetry, impedance spectroscopy).

Main Results:

• Aptamer-based biosensors demonstrate high specificity and sensitivity for thrombin detection.
• Optical and electrochemical sensors offer distinct advantages for thrombin quantification.
• These biosensors show promise for applications in thrombin analysis and disease diagnosis.

Conclusions:

• Aptamer-based biosensors represent a powerful tool for sensitive and specific thrombin quantification.
• Optical and electrochemical approaches provide versatile platforms for thrombin detection.
• Further development of these biosensors can significantly impact clinical diagnostics and research.