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Related Experiment Video

Updated: Jun 19, 2026

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA

Published on: September 13, 2012

Development of Specialized Microelectrode Arrays with Local Electroporation Functionality.

Andrea Kauth¹, Anne-Kathrin Mildner², Lena Hegel¹

¹Institute of Materials in Electrical Engineering 1, RWTH Aachen University, Sommerfeldstr. 18-24, 52074, Aachen, Germany.

Annals of Biomedical Engineering

|June 16, 2023

Summary

Researchers developed specialized microelectrode arrays (MEAs) for precise gene electrotransfer (GET) in cells. This technique uses localized electric fields for efficient gene delivery with high spatial resolution.

Keywords:

Electropermeabilization Gene electrotransfer Micro- and nanosystems Transfection

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Last Updated: Jun 19, 2026

High efficiency, Site-specific Transfection of Adherent Cells with siRNA Using Microelectrode Arrays MEA

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Published on: September 13, 2012

Microscale Vortex-assisted Electroporator for Sequential Molecular Delivery

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Published on: August 7, 2014

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

The Fabrication and Operation of a Continuous Flow, Micro-Electroporation System with Permeabilization Detection

Published on: January 7, 2022

Area of Science:

Biotechnology
Cell Biology
Bioengineering

Background:

Pulsed electric fields induce electropermeabilization (EP), allowing biomolecule entry into cells.
Gene electrotransfer (GET) utilizes EP for delivering therapeutic genes via plasmid DNA.
Micro-/nano-technologies offer enhanced spatial resolution and lower voltages for EP and GET compared to bulk methods.

Purpose of the Study:

To develop and characterize a specialized microelectrode array (MEA) for localized electropermeabilization (EP) of adherent cells.
To demonstrate the utility of MEAs for gene electrotransfer (GET) with high spatial resolution.

Main Methods:

Fabrication of a specialized MEA with flexible electrode and substrate material selection.
Electrochemical impedance spectroscopy to characterize MEA impedance with and without adherent cells.
Verification of local EP using a fluorophore dye in human embryonic kidney 293T cells.
Demonstration of GET by measuring subsequent green fluorescent protein expression.

Main Results:

The developed MEA enabled localized EP of adherent cells.
Electrochemical impedance spectroscopy confirmed the impact of cellular layers on MEA impedance.
Successful delivery of a fluorophore dye into cells was achieved, validating EP functionality.
Gene electrotransfer was successfully demonstrated, leading to observable green fluorescent protein expression.

Conclusions:

Specialized MEAs are effective tools for achieving high spatial resolution in gene electrotransfer.
The flexible manufacturing process allows for tailored MEA design for specific cellular applications.
This MEA-based approach offers a promising platform for localized and efficient gene delivery.