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Isolation, Processing and Analysis of Murine Gingival Cells
Published on: July 2, 2013
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Metaproteomics Characterizes the Human Gingival Crevicular Fluid Microbiome Function in Periodontitis
Xiaolian Xiao1,2,3, Xiaoping Xiao1,4, Yaoran Liu5
1Core Facility of Instrument, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, School of Basic Medicine, Peking Union Medical College, 5 Dong Dan San Tiao, Beijing 100005, China.
Journal of Proteome Research
|June 16, 2023
Summary
This study reveals key human and microbial protein changes in gingival crevicular fluid associated with periodontitis. Understanding these proteomic and metaproteomic shifts aids in deciphering the mechanisms of this common gum disease.
Area of Science:
- Oral biology
- Microbiology
- Proteomics
Background:
- Periodontitis is a major cause of adult tooth loss globally.
- The human and microbial protein profiles (proteome and metaproteome) in periodontitis are not well understood.
Purpose of the Study:
- To characterize the human proteome and metaproteome in gingival crevicular fluid of periodontitis patients.
- To identify differentially expressed proteins and microbial genera.
- To explore correlations between host and microbial protein expression.
Main Methods:
- Gingival crevicular fluid samples were collected from periodontitis and healthy subjects.
- Liquid chromatography and high-resolution mass spectrometry were used for protein identification.
- Differential expression and correlation analyses were performed.
Main Results:
- 570 human proteins were differentially expressed, linked to inflammation, cell death, cellular junctions, and fatty acid metabolism.
- 51 microbial genera were identified; 10 were upregulated and 11 downregulated in periodontitis.
- Microbial proteins involved in butyrate metabolism were upregulated.
- Host inflammatory and metabolic proteins correlated with microbial changes.
Conclusions:
- Gingival crevicular fluid proteome and metaproteome reflect periodontitis characteristics.
- Findings enhance understanding of periodontitis mechanisms.
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