Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modular lateral flow test systems for the competitive immunodetection of different classes of antibiotics in meat.

Analytical methods : advancing methods and applications·2026
Same author

Nanozyme-enhanced lateral flow immunochromatographic tests for the sensitive detection of <i>Francisella tularensis</i> cells in water samples.

Analytical methods : advancing methods and applications·2026
Same author

Discrimination of the S-isomer of ofloxacin using nanozyme-enhanced immunochromatographic analysis based on a secondary antibody-modified Au@Pd nanozyme.

Mikrochimica acta·2026
Same author

Application of Core-Shell Bimetallic Nanoparticles with Polydopamine-Assisted Nanogap in SERS-Based Lateral Flow Immunoassay of Prolactin.

Sensors (Basel, Switzerland)·2026
Same author

Widespread omission of aptamer-target binding verification in aptasensor development: Consequences for sensor performance and the need for a K<sub>d</sub> gate.

Biosensors & bioelectronics·2026
Same author

A farnesol-sensing triad in <i>Pseudomonas aeruginosa</i> drives interkingdom predation on <i>Candida albicans</i> via signal transduction.

Proceedings of the National Academy of Sciences of the United States of America·2026

Related Experiment Video

Updated: Jul 26, 2025

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

2.7K

Ligation-dependent Cas14a1-Activated biosensor for one-pot pathogen diagnostic.

Xiao Tan1, Xiufen Yang2, Yuefeng Qiao2

  • 1Department of Chemistry, Key Laboratory of Bioorganic Phosphorus Chemistry & Chemical Biology, Tsinghua University, Beijing, 100084, China.

Analytica Chimica Acta
|June 16, 2023
PubMed
Summary

We developed a rapid and sensitive bacterial RNA detection assay called Transcription-Amplified Cas14a1-Activated Signal Biosensor (TACAS). This all-in-one method offers high specificity for pathogen identification in various samples.

Keywords:
Cas14a1E. coliIsothermal one-pot assayLigation reactionPathogenic RNA detection

More Related Videos

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

377
Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.3K

Related Experiment Videos

Last Updated: Jul 26, 2025

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
09:03

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Published on: December 23, 2022

2.7K
Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
07:59

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

377
Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases
10:16

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

1.3K

Area of Science:

  • Molecular Biology
  • Biosensing Technology
  • Pathogen Detection

Background:

  • Accurate and rapid pathogen identification is crucial for timely diagnosis and treatment.
  • Existing nucleic acid diagnostic methods often require complex equipment and lengthy procedures.
  • There is a need for sensitive, specific, and user-friendly assays for bacterial RNA detection.

Purpose of the Study:

  • To establish an all-in-one, highly sensitive, and specific fluorescence-based biosensor for bacterial RNA detection.
  • To develop a rapid assay for pathogen identification using Cas14a1 activation.
  • To demonstrate the utility of the assay in real-world samples and for studying infection dynamics.

Main Methods:

  • Development of a Transcription-Amplified Cas14a1-Activated Signal Biosensor (TACAS) assay.
  • Utilizing a DNA promoter probe and reporter probe ligated via SplintR ligase upon target RNA hybridization.
  • Employing T7 RNA polymerase for transcription of the ligation product into Cas14a1 RNA activators, initiating a sustained isothermal cascade.
  • Cas14a1/sgRNA complex generating a fluorescence signal for detection.

Main Results:

  • Achieved a sensitive detection limit of 1.52 CFU mL⁻¹ for E. coli within 2 hours.
  • Demonstrated high specificity and excellent sensitivity in detecting bacterial RNA.
  • Successfully applied TACAS in contrived fish and milk samples, showing significant differentiation between infected and uninfected samples.
  • Explored E. coli colonization and transmission in vivo, enhancing understanding of infection mechanisms.

Conclusions:

  • The TACAS assay provides a powerful tool for rapid and sensitive bacterial RNA detection.
  • This all-in-one strategy offers excellent sensitivity and specificity for pathogen identification.
  • TACAS has potential applications in clinical diagnostics, food safety, and biological research for understanding infection dynamics.