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Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

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Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
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Labeling DNA Probes03:31

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Sanger Sequencing01:57

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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Drug Discovery: Overview01:26

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Drug discovery is a multifaceted process involving extensive screening, testing, and optimization of lead compounds to identify potential new drugs for therapeutic use. It combines several approaches, including screening large numbers of natural products, chemical modification of known active molecules, identification of new drug targets, and rational design based on biological mechanisms and drug-receptor structure. These approaches are carried out in both academic research laboratories and...
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DNA Isolation01:24

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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Updated: Jul 26, 2025

High-Density DNA and RNA microarrays - Photolithographic Synthesis, Hybridization and Preparation of Large Nucleic Acid Libraries
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Small-molecule discovery through DNA-encoded libraries.

Alexander A Peterson1,2,3, David R Liu4,5,6

  • 1Merkin Institute of Transformative Technologies in Healthcare, Broad Institute of MIT and Harvard, Cambridge, MA, USA.

Nature Reviews. Drug Discovery
|June 16, 2023
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Summary
This summary is machine-generated.

DNA-encoded library (DEL) technology accelerates the discovery of diverse bioactive small molecules for drug development. This review highlights recent DEL discoveries, their optimization, and clinical potential.

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Area of Science:

  • Medicinal Chemistry
  • Chemical Biology
  • Drug Discovery

Background:

  • Developing bioactive small molecules requires efficient methods to explore chemical diversity and identify target ligands.
  • DNA-encoded library (DEL) technology has become a prominent platform for small-molecule discovery over the last 15 years.
  • DELs offer significant advantages over traditional screening, including speed, scalability, and resource efficiency.

Purpose of the Study:

  • To review recent advancements and applications of DNA-encoded library technology in small-molecule discovery.
  • To showcase examples of bioactive small molecules identified through DEL screening.
  • To discuss the optimization and validation of DEL-discovered compounds for therapeutic potential.

Main Methods:

  • Leveraging DNA-encoded libraries for high-throughput screening of vast chemical spaces.
  • Identifying and characterizing novel small-molecule ligands against various biological targets.
  • Optimizing lead compounds for improved potency, selectivity, and pharmacokinetic properties.

Main Results:

  • DEL technology has successfully yielded diverse bioactive ligands for numerous therapeutically relevant targets.
  • Recent examples demonstrate the identification of promising small molecules through DEL screening.
  • Validated compounds show potential for further development into clinical applications.

Conclusions:

  • DNA-encoded library technology is a powerful and mature platform for discovering novel bioactive small molecules.
  • DELs enable efficient exploration of chemical diversity, leading to compounds with significant therapeutic promise.
  • The reviewed discoveries underscore the clinical applicability and ongoing impact of DELs in drug development.