Polyethylene glycol and chitosan functionalized manganese oxide nanoparticles for antimicrobial and anticancer...

Biocompatible polymer-functionalized magnetic nanoparticles could offer promising applications in biomedical sciences. We fabricated polymer functionalized tri-manganese tetra oxide (Mn₃O₄) nanoparticles with the co-precipitation method and an octahedral crystal structure having a crystallite size of 10-17 nm was identified via XRD analyses. The SEM graph depicted the non-uniform and smooth surface of PEG-functionalized Mn₃O₄ NPs as compared to Mn₃O₄ and chitosan-coated Mn₃O₄ NPs. Elemental composition in the prepared sample was examined by EDX analysis. Various modes such as MnO, MnOH, OH, symmetric, and anti-symmetric of CH₂ attached to the spectrum of Mn₃O₄ NPs were observed with FTIR analysis. The magnetization factor decreased and increase the coreacivity and retentivity of surface functionalized Mn₃O₄-NPs was calculated via VSM analysis. In-vitro bioassay, antibacterial activity was tested against Escherichiacoli, Bacillus cereus, and anti-fungal activities against two Fusarium strains indicated clear antimicrobial activities. The MTT assay to examine the anticancer activity against the MCF-7 cancer cell line was performed and the T₁ MRI contrast agent demonstrated that PEG-coated Mn₃O₄ NPs exhibited anti-cancer activities. We propose that surface-functionalized magnetic NPs used for the treatment of cancer by using a remote controlled process of hyperthermia therapy.