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Updated: Jul 26, 2025
Analysis of Gene Expression in Emerald Ash Borer Agrilus planipennis Using Quantitative Real Time-PCR
Published on: May 4, 2010
Transcriptome analysis of Euwallacea interjectus reveals differentially expressed unigenes related to developmental
Jiafeng Hu1, Chen Zhao1, Jiajin Tan1
1Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210036, China; College of Forestry, Nanjing Forestry University, Nanjing 210036, China.
Abstract:
Euwallacea interjectus (Curculionidae: Scolytinae) is an ambrosia beetle species in its early stages of research. Therefore, studying the related molecular mechanism associated with the development and egg laid is essential. Transcriptome sequencing was used in this study to compare the gene expression of the beetles at different developmental stages and female adults before and after oviposition. A total of 40,047 annotated unigenes were obtained. There were 4225 differentially expressed unigenes (DEUs) from larva to prepupa stage, 3651 DEUs between prepupa and pupa, 1675 DEUs generated from pupa to adult, and 4762 DEUs between females before and after oviposition. The most significant pathway differences between different development stages and before and after oviposition were selected through functional annotation of DEUs between different stages. Among them, there were many pathways related to protein metabolism including: neuroactive ligand-receptor interaction, endoplasmic reticulum and RNA transport. This study provides valuable information on the molecular regulation mechanism of development and the egg laid of E. interjectus.
