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Updated: Jul 26, 2025

Author Spotlight: Development of a Scaffold-Free Acoustic Assembly Method for High-Quality 3D Cell Spheroid Culture

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Newly Designed 3D-Printed Sonication Test Cell Optimized for In Vitro Sonication Experiments.

Martin Snehota¹, Marketa Kolarikova¹, Jaromir Vachutka¹

¹Department of Medical Biophysics, Faculty of Medicine and Dentistry, Palacky University Olomouc, Olomouc, Czech Republic.

Ultrasound in Medicine & Biology

|June 17, 2023

Summary

This summary is machine-generated.

Researchers developed novel sonication test cells to improve in vitro ultrasound experiments. These 3D-printed cells minimize ultrasound interaction, ensuring more accurate results for biological sample studies.

Keywords:

Optimization of sonication test cell Sonication experiments Therapeutic ultrasound Ultrasound field

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Area of Science:

Biophysics
Materials Science
Cell Biology

Background:

Precise control of ultrasound field parameters in in vitro sonication experiments is challenging.
Minimizing interaction between test cells and ultrasound is crucial for accurate experimental outcomes.

Purpose of the Study:

To outline an approach for constructing sonication test cells that minimize interaction with ultrasound.
To optimize test cell dimensions for consistent acoustic intensity.

Main Methods:

Determined optimal test cell dimensions using 3D-printed objects in a water sonication tank.
Measured local acoustic intensity variability within the test cell.
Assessed cytotoxicity of 3D printing materials using the MTT assay.

Main Results:

3D-printed polylactic acid sonication test cells were non-toxic.
A silicone membrane (HT-6240) minimally reduced ultrasound energy.
Achieved desired local acoustic intensity variability within the test cells.
Cell viability in the developed test cells was comparable to commercial plates.

Conclusions:

An effective approach for constructing sonication test cells that minimize ultrasound interaction has been developed.
The novel test cells offer a reliable platform for in vitro sonication studies.