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Characterization of bacteriophage vB_KleM_KB2 possessing high control ability to pathogenic Klebsiella pneumoniae

Qin Peng1, Zimeng Ma1, Qing Han1

  • 1Ministry of Education Key Laboratory for Ecology of Tropical Islands, College of Life Sciences, Hainan Normal University, Haikou, 571158, China.

Scientific Reports
|June 17, 2023
PubMed

Insights

A novel bacteriophage, vB_KleM_KB2, effectively targets multidrug-resistant Klebsiella pneumoniae. This phage shows high lytic activity and environmental stability, offering a promising alternative for combating resistant bacterial infections.

Area of Science:

  • Microbiology
  • Virology
  • Infectious Diseases

Background:

  • Klebsiella pneumoniae is a significant human pathogen.
  • The rise of multidrug-resistant (MDR) K. pneumoniae poses a major therapeutic challenge.
  • Bacteriophage therapy presents a potential solution for combating MDR bacteria.

Purpose of the Study:

  • To isolate and characterize a novel bacteriophage effective against MDR K. pneumoniae.
  • To evaluate the lytic activity, stability, and genetic novelty of the isolated bacteriophage.

Main Methods:

  • Isolation and characterization of bacteriophage vB_KleM_KB2 from clinical isolates.
  • Assessment of latent period and lysis time.
  • Determination of lytic activity at low multiplicity of infection.
  • Evaluation of environmental tolerances.
  • Genomic analysis of the bacteriophage.

Main Results:

  • A novel bacteriophage, vB_KleM_KB2, was isolated, targeting MDR K. pneumoniae.
  • The bacteriophage demonstrated a short latent period (10 min) and rapid lysis (60 min).
  • High lytic activity was observed, inhibiting bacterial growth at a low multiplicity of infection (0.001).
  • The bacteriophage exhibited significant environmental tolerances.
  • Genomic analysis revealed a novel sequence, suggesting a new bacteriophage genus.

Conclusions:

  • Bacteriophage vB_KleM_KB2 is a potent agent against MDR K. pneumoniae.
  • Its rapid lytic activity, stability, and unique genetic makeup make it a valuable addition to the phage library.
  • This bacteriophage offers a promising alternative for controlling diseases caused by MDR K. pneumoniae.

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