Related Experiment Video
Updated: Jul 26, 2025
Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae
Published on: October 6, 2017
Effects of PmDOME and PmSTAT knockdown on white spot syndrome virus infection in Penaeus monodon
Pasunee Laohawutthichai1,2, Thapanan Jatuyosporn1,2, Premruethai Supungul2,3
1Center of Excellence in Structural and Computational Biology, Department of Biochemistry, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand.
Abstract:
Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway plays an important role in antiviral immunity. This research reports the full-length DOME receptor gene in Penaeus monodon (PmDOME) and examines the effects of PmDOME and PmSTAT silencing on immune-related gene expressions in shrimp hemocytes during white spot syndrome virus (WSSV) infection. PmDOME and PmSTAT were up-regulated in shrimp hemocytes upon WSSV infection. Suppression of PmDOME and PmSTAT showed significant impacts on the expression levels of ProPO2 (melanization), Vago5 (interferon-like protein) and several antimicrobial peptides, including ALFPm3, Penaeidin3, CrustinPm1 and CrustinPm7. Silencing of PmDOME and PmSTAT reduced WSSV copy numbers and delayed the cumulative mortality caused by WSSV. We postulated that suppression of the JAK/STAT signaling pathway may activate the proPO, IFN-like antiviral cytokine and AMP production, resulting in a delay of WSSV-related mortality.
Insights
Silencing the Janus kinase/signal transducers and activators of transcription (JAK/STAT) pathway in shrimp boosted antiviral defenses. This reduced white spot syndrome virus (WSSV) levels and delayed shrimp mortality.
Area of Science:
- Immunology
- Molecular Biology
- Aquaculture
Background:
- The Janus kinase/signal transducers and activators of transcription (JAK/STAT) signaling pathway is crucial for antiviral immunity.
- Understanding its role in shrimp is vital for combating viral diseases like white spot syndrome virus (WSSV).
Purpose of the Study:
- To investigate the role of the DOME receptor (PmDOME) and PmSTAT in shrimp antiviral immunity.
- To analyze the impact of silencing PmDOME and PmSTAT on immune gene expression during WSSV infection.
Main Methods:
- Gene silencing of PmDOME and PmSTAT in Penaeus monodon hemocytes.
- Quantitative analysis of immune-related gene expression (ProPO2, Vago5, antimicrobial peptides).
- Monitoring of WSSV copy numbers and cumulative mortality.
Main Results:
- PmDOME and PmSTAT expression increased following WSSV infection.
- Silencing PmDOME and PmSTAT significantly altered immune gene expression, including melanization and antimicrobial peptides.
- Suppression of PmDOME and PmSTAT reduced WSSV loads and delayed mortality.
Conclusions:
- The JAK/STAT pathway negatively regulates antiviral responses in shrimp.
- Inhibiting the JAK/STAT pathway enhances proPO, interferon-like cytokine, and antimicrobial peptide production.
- Targeting the JAK/STAT pathway offers a potential strategy to mitigate WSSV impacts in shrimp aquaculture.
