Related Experiment Video

Updated: Jul 26, 2025

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer
04:20

Author Spotlight: Exploring the Role of FAM83A in Cervical Cancer

Published on: February 9, 2024

974

Transcription Factor E2F8 Promotes Cisplatin Resistance in Hepatocellular Carcinoma by Regulating DNA Damage via

Jianqiao Kong1, Song Xu1, Peng Zhang1

  • 1Department of General Surgery, Xiangyang No.1 People's Hospital, Hubei University of Medicine, Xiangyang, China.

International Journal of Toxicology
|June 18, 2023
PubMed

Insights

The E2F8/NUSAP1 pathway promotes cisplatin resistance in hepatocellular carcinoma (HCC) by inhibiting DNA damage repair. Targeting this axis could improve chemotherapy effectiveness in HCC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Cisplatin resistance is a major challenge in hepatocellular carcinoma (HCC) treatment.
  • DNA damage repair mechanisms are implicated in chemoresistance.
  • The role of Nucleolar and Spindle-Associated Protein 1 (NUSAP1) in HCC cisplatin resistance requires elucidation.

Purpose of the Study:

  • To investigate the molecular mechanism of NUSAP1 in regulating DNA damage and cisplatin tolerance in HCC.
  • To explore the regulatory relationship between E2F8 and NUSAP1 in HCC.
  • To assess the therapeutic potential of targeting the E2F8/NUSAP1 axis.

Main Methods:

  • Real-time quantitative PCR to detect mRNA expression of E2F8 and NUSAP1.
  • Chromatin immunoprecipitation (ChIP) and dual-luciferase reporter assays to confirm E2F8-NUSAP1 interaction.
  • Cell viability (CCK-8), cell cycle (flow cytometry), DNA damage (γ-H2AX, comet assay), and western blot analyses.

Main Results:

  • High mRNA expression of E2F8 and NUSAP1 was observed in HCC.
  • E2F8 directly binds to and regulates the promoter activity of NUSAP1.
  • NUSAP1 knockdown enhanced cisplatin-induced DNA damage and sensitivity in HCC.
  • Overexpression of E2F8 promoted cell cycle arrest, increased DNA damage, and enhanced cisplatin sensitivity by silencing NUSAP1.

Conclusions:

  • The E2F8/NUSAP1 axis plays a crucial role in regulating DNA damage and cisplatin resistance in HCC.
  • E2F8 enhances HCC chemoresistance by activating NUSAP1, which inhibits DNA damage.
  • This axis represents a potential therapeutic target to improve cisplatin efficacy in HCC.
Keywords:
DNA damageE2F8NUSAP1cisplatin resistancehepatocellular carcinoma

More Related Videos

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis09:58

Mapping the Structure-Function Relationships of Disordered Oncogenic Transcription Factors Using Transcriptomic Analysis

Published on: June 27, 2020

2.8K
Using the E1A Minigene Tool to Study mRNA Splicing Changes10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

4.9K

Related Concept Videos

DNA Damage can Stall the Cell Cycle02:37

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
9.2K
Nucleotide Excision Repair01:38

Nucleotide Excision Repair

DNA Distortion and Damage
Cells are regularly exposed to mutagens—factors in the environment that can damage DNA and generate mutations. UV radiation is one of the most common mutagens and is estimated to introduce a significant number of changes in DNA. These include bends or kinks in the structure, which can block DNA replication or transcription. If these errors are not fixed, the damage can cause mutations, which in turn can result in cancer or disease depending on which sequences are...
3.6K
DNA Damage Can Stall the Cell Cycle02:37

DNA Damage Can Stall the Cell Cycle

2.6K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
35.5K
Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.6K
Treatment Resistant Cancers02:56

Treatment Resistant Cancers

Cancer is the second leading cause of death in the United States. A cancer cell is genetically unstable and hence can mutate faster. They can also modify their microenvironment and escape immune surveillance. The difficulties in treating cancer are further compounded by the emergence of rapid resistance to anticancer drugs. The most common ways to attain resistance in cancer cells include alteration in drug transport and metabolism, modification of drug target, elevated DNA damage response, or...
3.4K