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Related Concept Videos

Centrifugation01:05

Centrifugation

2.4K
Centrifugation is a separation technique based on differences in density or size. It is commonly used to separate solids from aqueous interferents. During centrifugation, the sample is placed in centrifugation tubes and spun at high angular velocity, which allows centrifugal force to act differentially on the different densities or masses of the components. After spinning, the supernatant liquid is decanted. Depending on the specific application, either the pellet or the supernatant is retained...
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Subcellular Fractionation01:32

Subcellular Fractionation

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The homogenate obtained after cell lysis contains various membrane-bound organelles that can be further separated into pure fractions by subcellular fractionation. These isolates are used to study specific cellular components, analyze localized protein activity, and are even employed in diagnostics. Fractionation is typically achieved using centrifugation methods, the most common being density-gradient and differential centrifugation.
Differential Centrifugation
Differential centrifugation is...
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Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

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Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
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Automated Counterflow Centrifugal System for Small-Scale Cell Processing
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Cost-efficient cell clarification using an intensified fluidized bed centrifugation platform approach.

Martin Saballus1, Thomas Josef Filz1, David Pollard2

  • 1Sartorius, Corporate Research, Göttingen, Germany.

Biotechnology and Bioengineering
|June 19, 2023
PubMed
Summary

A novel single-use fluidized bed centrifugation (FBC) platform offers superior antibody recovery and cost-effectiveness for biopharmaceutical manufacturing. This technology addresses bottlenecks in current methods, supporting intensified bioprocesses and affordable patient treatments.

Keywords:
cell removalclarificationcost analysisfluidized bed centrifugehigh cell densitymonoclonal antibody

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Area of Science:

  • Biopharmaceutical Manufacturing
  • Process Engineering
  • Downstream Processing

Background:

  • Established biomanufacturing methods like disc stack centrifugation (DSC) and depth filtration (DF) present economic and technological limitations, including low biomass capacity and product loss.
  • Intensified biopharmaceutical production requires advanced cell clarification technologies to overcome existing bottlenecks.

Purpose of the Study:

  • To develop and evaluate a novel single-use (SU) fluidized bed centrifugation (FBC) platform with integrated filtration for biopharmaceutical cell clarification.
  • To assess the feasibility of FBC for high cell concentrations (>100x10^6 cells/mL) and its scalability to 200 L bioreactor volumes.
  • To compare the economic impact of the FBC approach against traditional DSC and DF technologies in industrial SU biomanufacturing.

Main Methods:

  • Development of a novel SU-based clarification platform combining FBC with integrated filtration.
  • Investigation of FBC performance at high cell concentrations (>100x10^6 cells/mL).
  • Scalability testing of FBC to a 200 L bioreactor scale for moderate cell concentrations.
  • Economic comparison of FBC with DSC and DF for annual mAb production volumes up to 500 kg.

Main Results:

  • Achieved low harvest turbidities (4 NTU) and high antibody recoveries (95%) in both high and moderate cell concentration trials.
  • Demonstrated FBC as the most cost-effective solution for annual mAb production below 500 kg.
  • Showed minimal impact of increasing cell concentrations on FBC process costs compared to established technologies.

Conclusions:

  • The novel SU-FBC platform effectively clarifies biopharmaceutical harvests with high cell densities, offering superior product recovery and low turbidity.
  • FBC presents a cost-effective and scalable solution for industrial biomanufacturing, particularly for intensified processes.
  • This technology supports the drive towards affordable patient treatments by overcoming limitations of current cell clarification methods.