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Related Concept Videos

Short-distance Transport of Resources02:12

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Short-distance transport refers to transport that occurs over a distance of just 2-3 cells, crossing the plasma membrane in the process. Small uncharged molecules, such as oxygen, carbon dioxide, and water, can diffuse across the plasma membrane on their own. In contrast, ions and larger molecules require the assistance of transport proteins due to their charge or size. Transport across membranes also occurs within individual cells, playing a variety of essential roles for the plant as a whole.
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Related Experiment Video

Updated: Jul 26, 2025

Author Spotlight: Direct Infusion Device for Molecule Delivery in Plants
08:52

Author Spotlight: Direct Infusion Device for Molecule Delivery in Plants

Published on: June 2, 2023

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Direct Infusion Device for Molecule Delivery in Plants.

Brian H Rhodes1, Richard R Stange1, Patrick Zagorski2

  • 1United States Department of Agriculture Agricultural Research Service U.S. Horticultural Research Laboratory.

Journal of Visualized Experiments : Jove
|June 19, 2023
PubMed
Summary
This summary is machine-generated.

A new, low-cost Direct Plant Infusion (DPI) device enables effective delivery of therapeutic compounds to citrus trees. This method aids in screening treatments for Huanglongbing (HLB) and its insect vector, Diaphorina citri.

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Area of Science:

  • Agricultural Science
  • Plant Pathology
  • Entomology

Background:

  • Traditional methods for testing plant therapeutics (foliar, soil-drench) suffer from variable uptake and environmental degradation.
  • Existing trunk injection methods often require expensive, proprietary equipment, limiting their accessibility for research.
  • A need exists for a simple, low-cost delivery system for screening Huanglongbing (HLB) treatments in small citrus trees.

Purpose of the Study:

  • To design and evaluate a novel Direct Plant Infusion (DPI) device for delivering compounds into the vascular tissue of citrus plants.
  • To assess the efficacy of the DPI device for delivering therapeutic compounds and evaluating their impact on Candidatus Liberibacter asiaticus (CLas) and Diaphorina citri (D. citri).

Main Methods:

  • A Direct Plant Infusion (DPI) device was fabricated using 3D-printing technology and readily available components.
  • Compound uptake was validated in citrus plants using a fluorescent marker (5,6-carboxyfluorescein-diacetate).
  • The DPI device delivered streptomycin to CLas-infected plants and imidacloprid to D. citri-infested plants to assess therapeutic effects.

Main Results:

  • The DPI device demonstrated uniform distribution of the fluorescent marker throughout the plant's vascular system.
  • Treatment with streptomycin via DPI resulted in a significant reduction in CLas titer in infected citrus plants.
  • Delivery of imidacloprid using the DPI device led to increased mortality in D. citri psyllids within 7 days.

Conclusions:

  • The developed DPI device offers a simple, low-cost, and effective method for delivering molecules into the vascular tissue of small citrus trees.
  • This system facilitates research and screening of therapeutic compounds for HLB and its vector, contributing to agricultural advancements.
  • The DPI device shows promise for improving the efficiency and accessibility of plant disease and pest management research.