Bioinformatic Prediction of Bulked Oligonucleotide Probes for FISH Using Chorus2

Fluorescence in situ hybridization (FISH) provides great conveniences for detection and visualization of specific genomic segments. Oligonucleotide (Oligo)-based FISH further broadened the applications in plant cytogenetics researches. High-specific single-copy oligo probes are essential for successful oligo-FISH experiments. Here, we introduce the bioinformatic pipeline to design genome-scaled single-copy oligos and filter repeat-related probes with Chorus2 software. Robust probes are accessible for both well-assembled genome and species without a reference genome based on this pipeline.