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Updated: Jul 26, 2025
Robust 3D DNA FISH Using Directly Labeled Probes
Published on: August 15, 2013
Visualization of Oligonucleotide-Based Probes Along Pseudochromosomes Using RIdeogram, KaryoploteR, and Circlize
1Faculty of Biology, Technische Universität Dresden, Dresden, Germany. Ludwig.Mann@tu-dresden.de.
Abstract:
Fluorescence in situ hybridization (FISH) using oligonucleotide-based probes is an innovative modification of classic FISH techniques, enabling karyotypic identifications. Here, we exemplarily describe the design and in silico visualization of oligonucleotide-based probes derived from the Cucumis sativus genome. Additionally, the probes are also plotted comparatively to the closely related Cucumis melo genome. The visualization process is realized in R using various libraries for linear or circular plots including RIdeogram, KaryoploteR, and Circlize.
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