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Firefly luciferin methyl ester illuminates the activity of multiple serine hydrolases
Innus Mohammad1, Kate L Liebmann1, Stephen C Miller1
1Department of Biochemistry and Molecular Biotechnology, UMass Chan Medical School, 364 Plantation St., Worcester, MA 01605, USA. Stephen.miller@umassmed.edu.
Abstract:
Firefly luciferin methyl ester is hydrolyzed by monoacylglycerol lipase MAGL, amidase FAAH, poorly-characterized hydrolase ABHD11, and hydrolases known for S-depalmitoylation (LYPLA1/2), not just esterase CES1. This enables activity-based bioluminescent assays for serine hydrolases and suggests that the 'esterase activity' responsible for hydrolyzing ester prodrugs is more diverse than previously supposed.
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