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Updated: Jul 26, 2025
Phage Phenomics: Physiological Approaches to Characterize Novel Viral Proteins
Published on: June 11, 2015
Complete Genome Sequencing of a Novel Pseudomonas aeruginosa Phage, UF_RH5
Abdolrazagh Hashemi Shahraki1, Majid Vahed1, Roshan Dinparastisaleh1
1Division of Pulmonary, Critical Care and Sleep, College of Medicine-Jacksonville, University of Florida, Gainesville, Florida, USA.
Abstract:
Here, we introduce UF_RH5, a novel lytic phage targeting clinically isolated Pseudomonas aeruginosa. It belongs to the Siphovirus morphology family, Septimatrevirus genus, with a 42,566-bp genome with a GC content of 53.60%, encoding 58 proteins. Under electron microscopy, UF_RH5 exhibits a length of 121 nm and a capsid size of 45 nm.
Insights
Researchers discovered UF_RH5, a new lytic bacteriophage effective against Pseudomonas aeruginosa. This Siphovirus family phage has a genome of 42,566 bp and specific morphological characteristics. It offers potential for combating bacterial infections.
Area of Science:
- Microbiology
- Virology
- Bacteriophage research
Background:
- Pseudomonas aeruginosa is a significant opportunistic pathogen causing various infections.
- The rise of antibiotic resistance necessitates alternative therapeutic strategies.
- Bacteriophages offer a promising alternative or adjunct to conventional antibiotics.
Purpose of the Study:
- To introduce and characterize UF_RH5, a novel lytic bacteriophage.
- To assess UF_RH5's efficacy against clinical isolates of Pseudomonas aeruginosa.
- To provide detailed genomic and morphological data of the new phage.
Main Methods:
- Isolation and characterization of a novel lytic bacteriophage, UF_RH5.
- Genomic sequencing and analysis of the UF_RH5 genome (42,566 bp, 53.60% GC content).
- Electron microscopy to determine UF_RH5's morphology (121 nm length, 45 nm capsid).
Main Results:
- UF_RH5 was identified as a novel lytic phage targeting Pseudomonas aeruginosa.
- The phage genome encodes 58 proteins.
- Morphological analysis revealed a Siphovirus family structure.
Conclusions:
- UF_RH5 represents a new potential therapeutic agent against Pseudomonas aeruginosa infections.
- Detailed characterization provides a foundation for further development and application.
- This discovery contributes to the growing field of phage therapy.
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