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Genome Sequence of Staphylococcus aureus Phage ESa2
Jordan T Bird1, Katie R Margulieux2, Kevin A Burke2
1Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, Arkansas, USA.
Microbiology Resource Announcements
|June 20, 2023
Summary
We sequenced the ESa2 phage, a virus targeting Staphylococcus aureus. This research details its genome, providing insights into phage-antibiotic therapies.
Area of Science:
- Microbiology
- Genomics
- Virology
Background:
- Staphylococcus aureus infections pose significant public health challenges.
- Bacteriophages offer a potential alternative or adjunct to antibiotics.
- Characterizing novel phages is crucial for developing phage-based therapies.
Purpose of the Study:
- To describe the genome of the lytic phage ESa2.
- To determine the genomic features of ESa2, a phage specific for Staphylococcus aureus.
Main Methods:
- Isolation of lytic phage ESa2 from environmental water.
- Whole-genome sequencing and bioinformatic analysis of the ESa2 genome.
Main Results:
- ESa2 is a member of the Herelleviridae family and Kayvirus genus.
- The ESa2 genome is 141,828 bp with 30.25% GC content.
- It contains 253 predicted protein-coding sequences, 3 tRNAs, and long terminal repeats.
Conclusions:
- The genomic characterization of ESa2 provides a foundation for its potential use in combating Staphylococcus aureus.
- Understanding phage genomes is essential for advancing phage therapy research.
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