Two-photon fluorescence probe for quantification of cyclosporine

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Materials Science

Background:

• Cyclosporine A (CsA) requires precise therapeutic drug monitoring due to its narrow therapeutic index.
• Accurate CsA quantification is crucial for optimizing treatment efficacy and minimizing toxicity.
• Existing methods for CsA detection can be time-consuming and complex.

Purpose of the Study:

• To develop a novel ratiometric fluorescent sensor for rapid and facile determination of CsA.
• To quantify CsA in human plasma samples using a two-photon fluorescence probe.
• To evaluate the sensor's performance for on-site detection applications.

Main Methods:

• Fabrication of a ratiometric fluorescent sensor using zeolitic imidazolate framework (ZIF-8) and norepinephrine-capped silver nanoparticles (AgNPs@NE).
• Utilizing the fluorescence quenching effect of CsA on the ZIF-8-AgNPs@NE probe.
• Optimizing sensor conditions and validating the method using human plasma samples.

Main Results:

• The developed probe demonstrated a linear response for CsA quantification in two ranges: 0.01–0.5 μg mL⁻¹ and 0.5–10 μg mL⁻¹.
• Achieved a low limit of detection of 0.007 μg mL⁻¹.
• Successfully applied the method to determine CsA concentrations in plasma samples from patients on oral CsA therapy.

Conclusions:

• The novel ratiometric fluorescent sensor offers a fast, facile, and sensitive platform for CsA determination.
• The developed method shows promise for on-site therapeutic drug monitoring of CsA.
• This approach can aid in optimizing CsA dosage and improving patient management.