Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Replicative Cell Senescence02:15

Replicative Cell Senescence

3.7K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
3.7K
Mesenchymal Stem Cells01:19

Mesenchymal Stem Cells

4.8K
Mesenchymal stem cells (MSCs) are adult stem cells that can differentiate into most connective tissue cell types, except for hematopoietic cells, depending upon the source of MSCs. For example, bone-marrow-derived MSCs (BM-MSCs) can differentiate into osteocytes, hepatocytes, and pancreatic and neuronal cells. MSCs can be isolated from various sources such as bone marrow, placenta, adipose tissue, teeth, and Wharton’s jelly, a gelatinous substance in the umbilical cord. The ease of their...
4.8K
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

3.2K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.2K
Tissue Renewal without Stem Cells01:23

Tissue Renewal without Stem Cells

1.8K
After cellular or tissue damage, the resident stem cells present in the human body can locally repair and regenerate the damaged tissue or organ. However, even though some tissues do not have stem cells, they can repair and regenerate with the help of pre-existing cells. For example, beta cells of the pancreas and hepatocytes of the liver can divide to renew and regenerate the tissue. Here, both cell division and cell death are well regulated by homeostasis.
However, failure of such a system...
1.8K
Renewal of Intestinal Stem Cells01:23

Renewal of Intestinal Stem Cells

2.6K
The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the...
2.6K
Renewal of Skin Epidermal Stem Cells01:12

Renewal of Skin Epidermal Stem Cells

2.6K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antibacterial, In Vitro Anti-Inflammatory and Anti-Acne Activities of <i>Patchouli</i> Essential Oil.

Pharmaceuticals (Basel, Switzerland)·2026
Same author

Single-cell analyses identify the ginseng embryonic protoderm as a native compartment for high-efficiency ginsenoside production.

Nature communications·2026
Same author

[Analysis of complicating factors in endodontic microsurgery].

Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology·2026
Same author

Developing WNT-derived bone anabolic peptides for skeletal aging and fracture by reconstructing thumb and index domains of WNT7B.

Nature biomedical engineering·2026
Same author

Impact of aerosol acidity on the heterogeneous chemistry of halogenated phenols.

Environmental pollution (Barking, Essex : 1987)·2026
Same author

Network toxicology and molecular docking identify BRCA1 as a functional target of the dietary carcinogen PhIP in colorectal cancer.

Discover oncology·2026

Related Experiment Video

Updated: Jul 26, 2025

De Novo Generation of Somatic Stem Cells by YAP/TAZ
13:05

De Novo Generation of Somatic Stem Cells by YAP/TAZ

Published on: May 7, 2018

9.1K

Releasing YAP dysfunction-caused replicative toxicity rejuvenates mesenchymal stem cells.

Fanyuan Yu1,2, Lin Yao1,2, Feifei Li1

  • 1State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, China.

Aging Cell
|June 21, 2023
PubMed
Summary

YAP dysfunction causes cell aging. Hippo-dependent YAP activity controls replicative senescence in mesenchymal stromal cells (MSCs) by regulating cell cycle and DNA repair, with mutations rejuvenating MSCs.

Keywords:
DNA damageagingmesenchymal stromal cellrejuvenationreplicative stress

More Related Videos

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells MSCs
06:20

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells MSCs

Published on: December 24, 2015

12.4K
Author Spotlight: Advancements in iPSCs and Genetic Disease Research
06:24

Author Spotlight: Advancements in iPSCs and Genetic Disease Research

Published on: October 20, 2023

1.2K

Related Experiment Videos

Last Updated: Jul 26, 2025

De Novo Generation of Somatic Stem Cells by YAP/TAZ
13:05

De Novo Generation of Somatic Stem Cells by YAP/TAZ

Published on: May 7, 2018

9.1K
Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells MSCs
06:20

Assessment of the Immunomodulatory Properties of Human Mesenchymal Stem Cells MSCs

Published on: December 24, 2015

12.4K
Author Spotlight: Advancements in iPSCs and Genetic Disease Research
06:24

Author Spotlight: Advancements in iPSCs and Genetic Disease Research

Published on: October 20, 2023

1.2K

Area of Science:

  • Cellular Biology
  • Molecular Biology
  • Aging Research

Background:

  • Hippo-independent YAP dysfunction impairs nuclear envelope integrity, leading to chronological aging in stromal cells.
  • Cellular senescence, particularly replicative senescence in mesenchymal stromal cells (MSCs), is a critical factor in aging.

Purpose of the Study:

  • To investigate the Hippo phosphorylation-dependent role of YAP in replicative senescence of MSCs.
  • To elucidate the downstream mechanisms of YAP in controlling cellular senescence and genome stability.

Main Methods:

  • Utilized in vitro expansion of MSCs to study replicative senescence.
  • Employed Hippo-off YAP mutations (YAPS127A/S381A) to assess YAP's function.
  • Analyzed YAP/TEAD's regulation of RRM2 expression and its impact on cell cycle progression.
  • Investigated YAP's control over replicative toxicity (RT) transcriptomics and DNA damage response/repair pathways.

Main Results:

  • Hippo phosphorylation reduces nuclear YAP, decreasing YAP protein levels and initiating replicative senescence.
  • YAP/TEAD governs RRM2 expression, facilitating G1/S transition and releasing replicative toxicity.
  • YAP modulates RT transcriptomics, delaying genome instability and enhancing DNA repair.
  • Hippo-off YAP mutations rejuvenated MSCs by maintaining cell cycle, reducing genome instability, and restoring regenerative capacity without tumorigenesis risks.

Conclusions:

  • YAP activity, modulated by Hippo phosphorylation, plays a crucial role in replicative senescence of MSCs through NE integrity-independent pathways.
  • Targeting YAP signaling offers a potential strategy for rejuvenating aged cells and restoring tissue function.