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Updated: Jul 26, 2025
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Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy
Published on: May 29, 2012
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Non-Invasive Detection, Precise Localization, and Perioperative Navigation of In Vivo Deep Lesions Using Transmission
Zongyu Wu1, Binge Deng1, Yutong Zhou1
1State Key Laboratory of Systems Medicine for Cancer, School of biomedical engineering, Shanghai Jiao Tong University, Shanghai, 200030, P. R. China.
Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|June 21, 2023
Summary
Ratiometric surface-enhanced transmission Raman spectroscopy (SETRS) enables non-invasive, precise localization of deep lesions. This novel technique guides surgeons in real-time, improving deep sentinel lymph node detection and biopsy navigation in vivo.
Area of Science:
- Biomedical Optics
- Molecular Imaging
- Surgical Navigation
Background:
- Optical techniques offer high sensitivity but struggle with deep tissue penetration for lesion detection.
- Accurate depth determination and localization of deep-seated lesions remain a clinical challenge.
- Transmission Raman Spectroscopy (TRS) shows promise but requires enhancement for in vivo applications.
Purpose of the Study:
- To develop and validate an in vivo ratiometric surface-enhanced transmission Raman spectroscopy (SETRS) system.
- To enable non-invasive detection, precise localization, and surgical navigation of deep lesions.
- To overcome the limitations of shallow tissue penetration in optical imaging modalities.
Main Methods:
- Utilized ultrabright surface-enhanced Raman spectroscopy (SERS) nanoparticles (10 pM detection limit).
- Employed a home-built, photosafe transmission Raman spectroscopy (TRS) setup.
- Developed a ratiometric SETRS strategy using ratios of multiple Raman spectral peaks for depth determination.
Main Results:
- Precisely determined phantom lesion depth in ex vivo rat tissues with an 11.8% mean absolute percentage error.
- Achieved accurate localization of a 6-mm-deep rat popliteal lymph node.
- Demonstrated successful perioperative navigation of in vivo lymph node biopsy in live rats under safe laser conditions.
Conclusions:
- Ratiometric SETRS provides a viable method for non-invasive localization and depth assessment of deep lesions.
- The developed system facilitates real-time surgical navigation for procedures like lymph node biopsy.
- This advancement represents a significant step towards the clinical translation of transmission Raman spectroscopy.
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