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Updated: Jul 26, 2025

Using the E1A Minigene Tool to Study mRNA Splicing Changes
Published on: April 22, 2021
Exon junction complex modulates the formation of the m6A epitranscriptome
Peng-Hui Song1, Li-Juan Ma2, Dong Yan1
11. State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai 200438, China.
Abstract:
N6-methyladenosine (m6A) is one of the most abundant chemical modifications in mRNA and plays essential roles in diverse physiological and pathological processes. m6A is highly enriched near stop codons and in long internal exons of mRNA, but the mechanism leading to this specific distribution has been unclear. Recently, three papers have solved this major problem by revealing that exon junction complexes (EJCs) act as m6A suppressors and shape the formation of the m6A epitranscriptome. Here, we briefly introduce the m6A pathway, elaborate the roles of EJC on the formation of m6A modification based on these results, and describe the effect of exon-intron structure on mRNA stability via m6A, which will help us better understand the latest progress in the m6A RNA modification field.
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