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Updated: Jul 26, 2025

A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons
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A Droplet-Based Microfluidic Approach and Microsphere-PCR Amplification for Single-Stranded DNA Amplicons

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PCR-based gene synthesis with overlapping unisense-oligomers asymmetric extension supported by a simulator for

Yasunori Nishida1, Kotetsu Kayama1, Taichi Endoh1

  • 1Department of Radiation Biology, School of Veterinary Medicine, Rakuno Gakuen University, Ebetsu, 069-8501, Japan.

Biotechniques
|June 21, 2023
PubMed

Keywords:
AESOEoptimization-free oligomer joiningsynthetic genesunisense joining oligomers

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