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Related Experiment Video

Updated: Jul 26, 2025

Massively Parallel Reporter Assays in Cultured Mammalian Cells

Massively Parallel Reporter Assays in Cultured Mammalian Cells

Published on: August 17, 2014

Fast construction of SARS-CoV-2 associated plasmid library using parallel cloning method.

Fan Meng¹, Jiao Guo¹, Shan Wang¹

¹Jujing-Chengdu Biotech, Chengdu, Sichuan, PR China.

|June 21, 2023

Summary

Researchers developed a high-throughput FastCloning platform to create a plasmid library for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) research. This efficient method rapidly generated 536 recombinant vectors, aiding COVID-19 studies.

Keywords:

COVID-19 Parallel cloning Plasmids library SARS-CoV-2

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CRISPR-based Shuttle Cloning: A High-throughput Cloning Method

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Published on: June 13, 2025

Area of Science:

Molecular Biology
Virology
Biotechnology

Background:

The COVID-19 pandemic, caused by SARS-CoV-2, necessitates robust research tools.
Plasmids containing viral sequences are crucial for studying SARS-CoV-2.
Existing methods for plasmid construction can be time-consuming and inefficient.

Purpose of the Study:

To develop a high-throughput platform for constructing SARS-CoV-2 associated plasmids.
To create a comprehensive plasmid library for diverse research applications.
To provide a rapid and efficient solution for researchers studying SARS-CoV-2.

Main Methods:

Utilized a FastCloning method for efficient plasmid construction.
Constructed a plasmid library incorporating 29 open reading frames (ORFs) of SARS-CoV-2.
Integrated 20 commonly used laboratory vectors into the plasmid library.

Main Results:

Generated a library of 536 unique recombinant vectors.
Achieved a high positive clone success rate of 92.4%.
Demonstrated the platform's capability for rapid and large-scale plasmid library construction.

Conclusions:

The FastCloning platform offers a rapid and efficient approach for SARS-CoV-2 plasmid library construction.
This resource accelerates research into SARS-CoV-2 and the COVID-19 pandemic.
The developed platform can be adapted for constructing plasmid libraries for other viral research.