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Related Concept Videos

Potentiometry: Membrane Electrodes01:15

Potentiometry: Membrane Electrodes

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Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at...
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Tuning Structural Defects on a Nominal Single-Layered Graphene Oxide Membrane for Selective Separation of

Dinesh K Behera1, Bratin Sengupta1, Fanglei Zhou2

  • 1Department of Chemical and Biological Engineering and RENEW Institute, The State University of New York, University at Buffalo, Buffalo, New York 14260, United States.

ACS Applied Materials & Interfaces
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Researchers developed a novel method to create ultrathin graphene oxide (GO) membranes. These membranes effectively separate similar-sized proteins by controlling structural defects, advancing separation technology.

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graphene oxidemembranenominal single layerplasmaseparation of biomolecules

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Area of Science:

  • Materials Science
  • Nanotechnology
  • Chemical Engineering

Background:

  • Two-dimensional (2D) materials offer potential for fabricating ultrathin, high-throughput separation membranes.
  • Graphene oxide (GO) is widely studied for membrane applications due to its hydrophilicity and functionality.
  • Creating single-layered GO membranes that utilize structural defects for molecular permeation remains a significant challenge.

Purpose of the Study:

  • To optimize the deposition methodology for fabricating nominal single-layered (NSL) graphene oxide membranes.
  • To utilize structural defects in GO flakes as the primary pathway for molecular transport.
  • To demonstrate tunable separation capabilities of NSL GO membranes for protein mixtures.

Main Methods:

  • A sequential coating methodology was employed to deposit NSL GO membranes with minimal GO flake stacking.
  • Oxygen plasma etching was used to tune the size of structural defects within the GO membrane.
  • The separation performance of the fabricated membranes was evaluated using model proteins like bovine serum albumin (BSA), lysozyme, and immunoglobulin G (IgG).

Main Results:

  • The study successfully fabricated NSL GO membranes where structural defects served as the dominant transport pathway.
  • Effective rejection of different model proteins was achieved by precisely tuning the structural defect size.
  • Similar-sized proteins (myoglobin and lysozyme) were separated with a separation factor of approximately 6 and 92% purity.

Conclusions:

  • The optimized deposition method enables the fabrication of NSL GO membranes with controllable structural defects.
  • These membranes demonstrate tunable pore sizes for effective separation of challenging molecular mixtures.
  • The findings present new possibilities for using GO flakes in fabricating advanced membranes for biotechnology applications.