Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Biological Methods for Microbial Control01:28

Biological Methods for Microbial Control

Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
Production of Biopesticides01:18

Production of Biopesticides

Biopesticides offer a sustainable alternative to chemical pesticides, utilizing microbial agents to control agricultural pests. Bacillus thuringiensis (Bt) is a widely employed bacterium known for its potent insecticidal activity. Bt biopesticides are favored for their specificity to insect pests, minimal environmental impact, and natural degradability.Mechanism of Bt Toxin Action Bt produces insecticidal crystal (Cry) proteins during its sporulation phase. These proteins form parasporal...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Capsular K-antigen Coats Outer Membrane Vesicles of <i>Porphyromonas gingivalis</i>.

bioRxiv : the preprint server for biology·2026
Same author

Pathogenicity and virulence of <i>Erwinia amylovora</i>.

Virulence·2026
Same author

Author Correction: RejuAgro A as an antimicrobial for fire blight control of pome fruits and beyond.

Nature communications·2026
Same author

RejuAgro A as an antimicrobial for fire blight control of pome fruits and beyond.

Nature communications·2026
Same author

Microbial community succession and dynamics during the season-long development of apple fruit (<i>Malus</i> × <i>domestica</i>).

Microbiology spectrum·2026
Same author

<i>Porphyromonas gingivalis</i> sphingolipids impair neutrophil function and promote bacterial survival.

Journal of oral microbiology·2025

Related Experiment Video

Updated: Jun 22, 2026

Transmitting Plant Viruses Using Whiteflies
10:22

Transmitting Plant Viruses Using Whiteflies

Published on: November 8, 2013

28.9K

Erwinia amylovora Type III Secretion System Inhibitors Reduce Fire Blight Infection Under Field Conditions.

Xiaochen Yuan1, George W Sundin2, Quan Zeng3

  • 1Department of Plant Pathology, Entomology and Microbiology, Iowa State University, Ames, IA 50011.

Phytopathology
|June 21, 2023
PubMed
Summary

Type III secretion system (T3SS) inhibitors, like TS108, effectively manage fire blight disease in apples and pears. These compounds target bacterial virulence, significantly reducing blossom blight incidence in field trials.

Keywords:
Erwinia amylovoraT3SS inhibitorfire blightplant phenolics

More Related Videos

Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests
09:42

Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests

Published on: September 28, 2021

9.4K
Author Spotlight: Eco-Friendly Pest Management Using Entomopathogenic Fungi Against Mustard Aphids
08:28

Author Spotlight: Eco-Friendly Pest Management Using Entomopathogenic Fungi Against Mustard Aphids

Published on: July 21, 2023

1.9K

Related Experiment Videos

Last Updated: Jun 22, 2026

Transmitting Plant Viruses Using Whiteflies
10:22

Transmitting Plant Viruses Using Whiteflies

Published on: November 8, 2013

28.9K
Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests
09:42

Isolation and Selection of Entomopathogenic Fungi from Soil Samples and Evaluation of Fungal Virulence against Insect Pests

Published on: September 28, 2021

9.4K
Author Spotlight: Eco-Friendly Pest Management Using Entomopathogenic Fungi Against Mustard Aphids
08:28

Author Spotlight: Eco-Friendly Pest Management Using Entomopathogenic Fungi Against Mustard Aphids

Published on: July 21, 2023

1.9K

Area of Science:

  • Plant Pathology
  • Bacteriology
  • Molecular Biology

Background:

  • Fire blight, caused by *Erwinia amylovora*, is a significant economic threat to apple and pear production globally.
  • The type III secretion system (T3SS) is crucial for *E. amylovora*'s virulence.
  • Type III secretion system inhibitors offer a novel strategy for managing bacterial plant diseases by targeting virulence without impacting bacterial growth.

Purpose of the Study:

  • To investigate the mode of action of the T3SS inhibitor TS108 against *Erwinia amylovora*.
  • To evaluate the efficacy of TS108 in managing fire blight under field conditions.

Main Methods:

  • In vitro analysis of gene expression in *E. amylovora* treated with TS108.
  • Posttranscriptional regulation studies involving the CsrB small RNA and *hrpS* gene.
  • Species-specificity assessment of TS108 on T3SS expression in *Dickeya dadantii* and *Pseudomonas aeruginosa*.
  • Five independent field experiments across four US states (MI, NY, OR, CT) from 2015-2022.

Main Results:

  • TS108 repressed the expression of T3SS regulon genes, including *hrpL*, in *E. amylovora*.
  • TS108 negatively regulated CsrB posttranscriptionally, leading to repression of *hrpS* and subsequently *hrpL*.
  • TS108 demonstrated species-specific inhibition, affecting only *E. amylovora*'s T3SS.
  • Field trials showed up to 96.7% reduction in blossom blight incidence compared to untreated controls.

Conclusions:

  • TS108 functions by downregulating the *E. amylovora* T3SS via the CsrB/HrpS/HrpL regulatory pathway.
  • TS108 is a promising, species-specific T3SS inhibitor for effective fire blight management.
  • The findings support the development of T3SS inhibitors as a sustainable agricultural strategy.