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Updated: Jul 26, 2025

Genome-wide Gene Deletions in Streptococcus sanguinis by High Throughput PCR
Published on: November 23, 2012
pBFK, a new thermosensitive shuttle vector for Streptococcus pyogenes gene deletion by homologous recombination
S Boukthir1,2, P Gaudu3, A Faili2,4,5
1CHU de Rennes, Service de Bactériologie-Hygiène Hospitalière, Rennes, France.
Abstract:
Streptococcus pyogenes, or Group A Streptococcus (GAS), is a major human pathogen for which genetic manipulation remains an ongoing challenge. We created a new temperature-sensitive plasmid pBFK expressing spectinomycin resistance adapted to homologous recombination procedure to perform a complete gene deletion in GAS. Herein the mutagenesis strategy with pBFK was performed in a highly virulent GAS emm3 genotype.
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