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Rapid Identification of Pathogens01:25

Rapid Identification of Pathogens

MALDI-TOF MS has transformed clinical microbiology by offering a rapid and reliable method for pathogen identification. The traditional approach to microbial identification typically involves time-consuming culture techniques and biochemical tests, which can delay the initiation of appropriate antimicrobial therapy. MALDI-TOF MS avoids these delays by using characteristic ribosomal protein mass patterns of microbial cells, enabling accurate species-level identification within minutes.Principle...

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Updated: Jun 24, 2026

PTR-ToF-MS Coupled with an Automated Sampling System and Tailored Data Analysis for Food Studies: Bioprocess Monitoring, Screening and Nose-space Analysis
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Implementing the Use of Collision Cross Section Database for Phycotoxin Screening Analysis.

Maria Mar Aparicio-Muriana1,2, Renato Bruni1, Francisco J Lara2

  • 1Department of Food and Drug, University of Parma, Parco Area delle Scienze 17/A, Parma 43124, Italy.

Journal of Agricultural and Food Chemistry
|June 22, 2023
PubMed
Summary

Blue-green algae (BGA) supplements may contain harmful cyanotoxins. This study developed a new database using UHPLC-TWIMS-QTOF to identify these toxins, successfully detecting them in commercial BGA products.

Keywords:
BGA dietary supplementsCCS databasecollision cross sectioncyanotoxinsion mobility mass spectrometryphycotoxins

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Area of Science:

  • Food Safety
  • Analytical Chemistry
  • Mass Spectrometry

Background:

  • Growing consumption of blue-green algae (BGA) dietary supplements necessitates robust food safety assessments.
  • Concerns exist regarding the potential presence of harmful cyanotoxins in BGA products.
  • Liquid chromatography coupled with ion mobility spectrometry is a powerful technique for analyzing complex food matrices.

Purpose of the Study:

  • To establish the first comprehensive database of TWIMS-derived collision cross sections (TWCCS_N2) for phycotoxins.
  • To validate the use of UHPLC-TWIMS-QTOF for the reliable detection and identification of cyanotoxins in BGA supplements.
  • To assess the food safety of commercial BGA dietary supplements regarding cyanotoxin contamination.

Main Methods:

  • Ultra-high-performance liquid chromatography coupled to traveling wave ion mobility spectrometry/quadrupole time-of-flight mass spectrometry (UHPLC-TWIMS-QTOF) was utilized.
  • A database of 81 adducts for 20 cyanotoxins and 1 marine toxin was created, including accurate m/z, retention times, and TWCCS_N2 values.
  • Matrix independence and reproducibility of TWCCS_N2 measurements were evaluated.

Main Results:

  • The TWCCS_N2 database for phycotoxins was successfully established.
  • Accurate analytical data (m/z, retention time, TWCCS_N2) were generated for numerous toxin adducts in both ESI+ and ESI- modes.
  • Cyanotoxins were confidently identified in five out of 19 commercial BGA dietary supplements analyzed.

Conclusions:

  • UHPLC-TWIMS-QTOF is a reliable method for screening phycotoxins in food supplements.
  • The developed TWCCS_N2 database enhances the identification capabilities for cyanotoxins.
  • The findings highlight the importance of monitoring cyanotoxin contamination in BGA-based dietary products.