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Updated: Jul 26, 2025

Qualitative Identification of Carboxylic Acids, Boronic Acids, and Amines Using Cruciform Fluorophores
Published on: August 19, 2013
Benzophenoxazine-based colorimetric and fluorescent probe for highly sensitive detection of amines and food freshness
Hongjin Wang1, Wenzhu Yin2, Hui Ma3
1College of Chemistry and Environmental Science, Yili Normal University, Yining 835000, PR China; State Key Laboratory of Coordination Chemistry, State Key Laboratory of Analytical Chemistry for Life Science, Jiangsu Key Laboratory Advanced Organic Materials, School of Chemistry and Chemical Engineering, Nanjing University, Nanjing 210023, PR China.
Abstract:
In this work, we reported a chromogenic and near infrared (NIR) region fluorogenic dual-channel probe NRB, which could visually detect gaseous amines with high sensitivity (eg. 50 and 17 ppt for methylamine (MeNH2) via naked eyes and fluorescence spectrometer respectively). It exhibited a wide fluorescent emission band extending to the NIR region with a peak at 615 nm when stimulated by the MeNH2 solution. The plausible sensing mechanism was proved by mass spectrometry, where the reaction process was based on a nucleophilic substitution between the probe and amines rather than the ester group hydrolysis. Furthermore, NRB was successfully applied to monitor the food freshness (seafood and meat food), because of its low cytotoxicity and excellent photophysical properties. It was worth mentioning that real time monitoring for food quality can be realized visually by using a 365 nm UV lamp. In addition, the probe was stable during the quality guarantee period for perishable packaged food. It was believed that the applied experiments have demonstrated the value of this probe in the practical applications for food safety.
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