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Updated: Jul 26, 2025
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Published on: March 5, 2022
Single-cell m6A mapping in vivo using picoMeRIP-seq
Yanjiao Li1,2, Yunhao Wang3,4, Maria Vera-Rodriguez1,5
1Department of Microbiology, Oslo University Hospital, Rikshospitalet, Oslo, Norway.
Abstract:
Current N6-methyladenosine (m6A) mapping methods need large amounts of RNA or are limited to cultured cells. Through optimized sample recovery and signal-to-noise ratio, we developed picogram-scale m6A RNA immunoprecipitation and sequencing (picoMeRIP-seq) for studying m6A in vivo in single cells and scarce cell types using standard laboratory equipment. We benchmark m6A mapping on titrations of poly(A) RNA and embryonic stem cells and in single zebrafish zygotes, mouse oocytes and embryos.
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