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Related Concept Videos

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RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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Updated: Jul 26, 2025

A Nonsequencing Approach for the Rapid Detection of RNA Editing
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Profiling dynamic RNA-protein interactions using small-molecule-induced RNA editing.

Kyung W Seo1, Ralph E Kleiner2

  • 1Department of Chemistry, Princeton University, Princeton, NJ, USA.

Nature Chemical Biology
|June 22, 2023
PubMed
Summary
This summary is machine-generated.

We developed TRIBE-ID to track dynamic RNA-protein interactions. This method reveals how stress granules strengthen existing RNA-protein binding and identifies new binding events, aiding RBP function studies.

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Cell Biology

Background:

  • RNA-binding proteins (RBPs) are crucial for cellular processes.
  • Understanding dynamic RNA-protein interactions is key to RBP function.
  • Existing methods lack temporal control for studying these interactions.

Purpose of the Study:

  • To develop a facile strategy for quantifying state-specific RNA-protein interactions.
  • To profile dynamic RNA-protein interactions with temporal control.
  • To characterize small-molecule modulators of RBP-RNA binding.

Main Methods:

  • Developed Targets of RBPs Identified by Editing Induced Through Dimerization (TRIBE-ID).
  • Utilized rapamycin-mediated chemically induced dimerization and RNA editing.
  • Applied TRIBE-ID to G3BP1 and YBX1 under normal and oxidative stress conditions.

Main Results:

  • Stress granule formation strengthens pre-existing RNA-protein interactions.
  • Stress granule formation induces new RNA-protein binding events.
  • G3BP1 stabilizes its targets independently of stress granule formation.

Conclusions:

  • TRIBE-ID provides a general approach to profile dynamic RNA-protein interactions.
  • The method allows for temporal control in cellular contexts.
  • Characterized small-molecule modulators of G3BP1-RNA binding.