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Photon and Proton irradiation in Patient-derived, Three-Dimensional Soft Tissue Sarcoma Models
Siyer Roohani1,2, Jürgen Loskutov3, Jens Heufelder4,5
1Charité - Universitätsmedizin Berlin, corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin, Department of Radiation Oncology, Augustenburger Platz 1, 13353, Berlin, Germany. siyer.roohani@charite.de.
BMC Cancer
|June 22, 2023
Summary
Patient-derived 3D sarcoma cell cultures reveal significant differences in radiosensitivity between soft tissue sarcoma (STS) subtypes. These models show potential for developing personalized radiotherapy for STS patients.
Area of Science:
- Oncology
- Radiation Oncology
- Biomedical Engineering
Background:
- Current soft tissue sarcoma (STS) radiotherapy lacks subtype specificity, using a one-size-fits-all approach.
- Patient-derived 3D cell cultures offer a novel platform for subtype-specific STS research.
- This study introduces a methodology for irradiating STS 3D cultures and assessing radiosensitivity.
Purpose of the Study:
- To establish a reproducible method for irradiating patient-derived STS 3D cell cultures.
- To investigate radiosensitivity differences between undifferentiated pleomorphic sarcoma (UPS) and pleomorphic liposarcoma (PLS) subtypes.
- To compare the efficacy of photon and proton radiation on STS cell viability.
Main Methods:
- Two STS patient-derived 3D cell cultures (UPS and PLS) were irradiated with photons or protons.
- Irradiation doses ranged from 0 Gy (sham) to 16 Gy in single fractions.
- Cell viability was assessed at four and eight days post-irradiation.
Main Results:
- Significant differences in tumor cell viability were observed between UPS and PLS at various radiation doses.
- Both photon and proton irradiation demonstrated dose-dependent cell killing.
- Radiation's cell-killing effect persisted for eight days post-irradiation.
Conclusions:
- Patient-derived 3D STS cell cultures exhibit distinct radiosensitivity profiles, reflecting clinical heterogeneity.
- Photon and proton radiation showed comparable dose-dependent efficacy in killing STS cells.
- 3D STS cell cultures are a promising tool for advancing personalized, subtype-specific radiotherapy research.
