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Updated: Jul 25, 2025

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Protocol for Human Blastoids Modeling Blastocyst Development and Implantation
Published on: August 10, 2022
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Generation of Human Blastoids from Naive Pluripotent Stem Cells
Yulei Wei1,2, Kun Liu3,4, Carlos A Pinzon-Arteaga1
1Department of Molecular Biology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Methods in Molecular Biology (Clifton, N.J.)
|June 23, 2023
Abstract
None:
Under certain culture conditions, naive human pluripotent stem cells can generate human blastocyst-like structures (called human blastoids). Human blastoids serve as an accessible model for human blastocysts and are amenable for large-scale production. Here, we describe a detailed step-by-step protocol for the robust and high-efficient generation of human blastoids from naive human pluripotent stem cells.

