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Updated: Jul 25, 2025

The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior
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FOXJ3 Regulates Cell Proliferation and Motility Through Modulating Snail Expression in Breast Cancer Cells

Yulyeong Seo1,2, Jimin Lee1,2, Jinho Kim1,2

  • 1Department of Biochemistry and Molecular Biology, University of Ulsan College of Medicine, Seoul, Republic of Korea.

Anticancer Research
|June 23, 2023
PubMed
Abstract

Insights

Forkhead Box J3 (FOXJ3) is overexpressed in breast cancer and influences cell proliferation, migration, and invasion. Targeting FOXJ3 may offer a new therapeutic strategy for breast cancer treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cancer Research

Background:

  • Breast cancer is a leading cause of cancer-related deaths globally.
  • Current therapies have limited impact on patient survival and quality of life.
  • The role of Forkhead Box J3 (FOXJ3) in breast cancer remains uncharacterized.

Purpose of the Study:

  • To investigate the role of FOXJ3 in breast cancer development.
  • To determine FOXJ3's influence on cancer cell migration and invasion.
  • To explore FOXJ3 as a potential therapeutic target.

Main Methods:

  • Analysis of FOXJ3 expression in patient tissues and cell lines.
  • Loss-of-function and gain-of-function studies in MDA-MB-231 and MCF7 cells.
  • Assays for cell proliferation, migration, invasion, and Snail expression.

Main Results:

  • FOXJ3 is overexpressed in breast cancer tissues and cell lines.
  • Modulating FOXJ3 affects breast cancer cell proliferation, migration, and invasion.
  • FOXJ3 regulates Snail expression, a key factor in epithelial-mesenchymal transition.

Conclusions:

  • FOXJ3 plays a significant role in breast cancer progression.
  • FOXJ3 influences cell proliferation, migration, and invasion via Snail regulation.
  • FOXJ3 represents a potential therapeutic target for breast cancer.
Keywords:
Breast cancerFOXJ3Snailinvasionmigration

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