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Related Experiment Video

Updated: Jul 25, 2025

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
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Highly sensitive, modification-free, and dynamic real-time stereo-optical immuno-sensor.

Xuyang Zhao1, Zhihe Guo1, Yi Zhou1

  • 1The Key Laboratory of Micro and Nano Photonic Structures, Department of Optical Science and Engineering, Fudan University, Shanghai, 200438, China.

Biosensors & Bioelectronics
|June 23, 2023
PubMed
Summary

This study introduces a novel optical biosensor using a microbubble optofluidic channel for modification-free, highly sensitive detection of biomolecules. The "stereo-sensor" achieves real-time analysis with unprecedented specificity and resolution.

Keywords:
BiosensingFabry-Pérot microcavityMicrobubbleProtein conformationStereo sensor

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Area of Science:

  • Optofluidics
  • Biosensing
  • Optical Physics

Background:

  • Modification-free biosensing is crucial for real-time molecular analysis but faces challenges with sensitivity and specificity.
  • Existing optical biosensors often require surface pre-modification or fluorescent labeling, limiting their performance.
  • Low quality factor (Q) and sensitivity are common issues in current optical biosensor designs.

Purpose of the Study:

  • To develop a novel optical sensor prototype for modification-free biosensing.
  • To achieve high specificity and sensitivity in detecting biomolecules.
  • To enable real-time, integrated molecular analyses with improved performance.

Main Methods:

  • Integration of a microbubble optofluidic channel within a Fabry-Pérot cavity.
  • Utilizing intra-cavity lensing effect of the microbubble to tailor the light field.
  • Experimental characterization of the sensor's optical properties and detection capabilities.

Main Results:

  • Achieved a high Q-factor (∼10^5), small mode volume, and high light energy density.
  • Demonstrated ultrahigh refractive index (RI) sensitivity (679 nm/RIU) and resolution (∼10^-7 RIU).
  • Successfully detected low concentrations of human IgG (5 fg/mL) and HSA (0.5 pg/mL) without probe pre-modification.

Conclusions:

  • The developed "stereo-sensor" offers a modification-free approach for highly sensitive and specific biomolecular detection.
  • The sensor design enables continuous, real-time, and multiplexed operations for dynamic biomolecular analyses.
  • This technology holds significant potential for point-of-care diagnostics and in vitro/in vivo monitoring.