The shock of benzalkonium chloride on aerobic granular sludge system and its microbiological mechanism

Quaternary ammonium compounds (QACs) are a kind of biocides and surfactants widely used around the world and wastewater treatment systems were identified as its largest pool. QACs could significantly inhibit microbial activity in biological treatment. Aerobic granular sludge (AGS) is an emerging wastewater biological treatment technology with high efficiency and resistance, but it is still unclear if AGS system could tolerate QACs shock. In this study, a typical QAC (benzalkonium chloride (BACC12)) was selected to investigate its effect on AGS system. Results indicate that BAC could inhibit the pollutants removal performance of AGS system, including COD, NH₄⁺-N and PO₄^3- in the short term and the inhibition ratio had positive correlation with BAC concentration. However, AGS system could gradually adapt to the BAC stress and recover its original performance. BAC shock could destroy AGS structure by decreasing its particle size and finally leading to particle disintegration. Although AGS could secret more EPS to resist the stress, BAC still had significant inhibition on cell activity. Microbial community analysis illustrated that after high BAC concentration shock in short term, Thauera decreased significantly while Flavobacterium became the dominant genus. However, after the performance of AGS system recovered the dominant genus returned to Thauera and relevant denitrifiers Phaeodactylibacter, Nitrosomonas and Pseudofulvimonas also increased. The typical phosphorous removal microorganism Rubrivivax and Leadbetterella also showed the similar trend. The variation of denitrification and phosphorus removal microbial community was consistent with AGS system performance indicating the change of functional microorganism played key role in the AGS response to BAC stress.