A dual-modal biosensor coupling cooperative catalysis strategy for sensitive detection of AFB₁ in agri-products

Herein, the cooperative catalysis effect between nanocomposite (AgPd NPs/POD-M/PEI-rGO) and horseradish peroxidase (HRP) was applied for the fast and sensitive detection of aflatoxin B1 (AFB₁). Upon specific and competitive binding of HRP@DNA and AFB₁ to cDNA, the working electrode presented different catalytic capacities for supporting electrolytes (TMB and H₂O₂). In the redox mechanism of TMB and H₂O₂, HRP and nanocomposite effectively catalyzed the oxidization of TMB to form the one-electron oxidation intermediate TMB+, and contributed the electrical signals and absorbance signals. Electrochemistry and colorimetric analyses were successfully realized for AFB₁ detection with 0.2 pg/mL and 8 pg/mL of detection limits, respectively, which is much lower than that of traditional HPLC methods. Overall, this method had significant reliability and sensitivity, offering a promising potential for conveniently evaluating the quality of agri-products polluted with AFB₁. Moreover, this approach provides a new idea for fast and accurate detection of mycotoxin.