Biodegradable and hemocompatible alginate/okra hydrogel films with promising stability and biological attributes

Currently, combinations of natural polymers and semi-synthetic biomolecules have gained attention for food-packaging, drug delivery, coatings, and biomedical applications. In this work, cross-linking property of two biopolymers was employed for the fabrication of hydrogel films. Sodium alginate (SAlg) and Okra gel (OkG) were used in different ratios (95:05, 75:25 and 85:15) to synthesize hydrogel films by solvent-casting method. Formation of the films was confirmed by FTIR and Raman techniques which specified the interaction between biomolecules of SAlg and OkG. XRD pattern has shown the presence of both amorphous and micro-crystalline phases in the hydrogel films and SEM studies have shown porosity, amorphousness and agglomerated morphology. TGA and DSC analyses revealed degradation of the film at 420 °C and stability studies using PBS buffer indicated stability and hydrophilic nature of hydrogel films. In-vitro degradation test was also performed for 10 weeks through the incubation of hydrogel-films in simulated body fluid and the effect of pH and temperature was also studied. Results have shown worth-some influence of okra gel on the fabricated films. Hemolytic and antioxidant activities of the gels were also determined and being non-toxic, all these ratios were found suitable for biomedical applications; especially 85:15 have shown maximum potential.