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Updated: Jul 25, 2025

Microbiologically Induced Calcite Precipitation Mediated by Sporosarcina pasteurii
Published on: April 16, 2016
Growing bio-tiles using microbially induced calcium carbonate precipitation
Emma Jane Horn1, Robert Huddy2, Dyllon Garth Randall1
1Civil Engineering Department, University of Cape Town, Cape Town 7700, South Africa; Future Water Institute, University of Cape Town, Cape Town 7700, South Africa.
Researchers developed eco-friendly bio-tiles using microbially induced calcium carbonate precipitation (MICP). This sustainable method offers a low-temperature alternative to conventional ceramic tiles, reducing carbon emissions and meeting high strength standards.
Area of Science:
- Materials Science
- Biotechnology
- Sustainable Construction
Background:
- Conventional ceramic tile production requires high temperatures (>1000°C), contributing to significant global carbon emissions.
- There is a need for sustainable building materials that reduce environmental impact and energy consumption.
Purpose of the Study:
- To investigate the growth of bio-tiles using microbially induced calcium carbonate precipitation (MICP) as a sustainable alternative to ceramic tiles.
- To optimize parameters for producing bio-tiles with mechanical properties comparable to conventional tiles.
Main Methods:
- Utilized the ureolytic activity of Sporosarcina pasteurii for MICP.
- Employed a novel submersion method with custom molds for bio-tile growth.
- Optimized key parameters including ureolytic activity, cementation solution concentration, particle size distribution, solution volume, and magnesium supplementation.
Main Results:
- Achieved bio-tile dimensions of 100 × 100 × 10 mm.
- Produced bio-tiles with a breaking strength of 637 N ± 60 N and a modulus of rupture of 13.0 N/mm² ± 2.3 N, exceeding conventional standards.
- Identified an optimal CaCO₃ precipitation rate constant (0.11-0.18 day⁻¹) for bio-tile production.
Conclusions:
- The optimized MICP process yields bio-tiles that meet or exceed the strength requirements of conventional ceramic tiles.
- The developed bio-tile manufacturing technique is scalable, easy to operate, and environmentally friendly, operating at room temperature.
- This study presents a viable, low-carbon footprint alternative for tile production.
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