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On Target: An Intrapulmonary Transplantation Method for Modelling Lung Tumor Development in its Native
Jackson A McDonald1,2, Leanne Scott1, Jessica Van Zuylekom3
1ACRF Cancer Biology and Stem Cells Division, The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, Australia.
Methods in Molecular Biology (Clifton, N.J.)
|June 24, 2023
Summary
Developing an intrapulmonary transplantation method allows lung cancer cells to grow in their native microenvironment. This technique, combined with PET imaging, enables precise tracking of tumor growth and spread in mouse models.
Area of Science:
- Oncology
- Translational Medicine
- Preclinical Research
Background:
- Accurate in vivo lung cancer models are essential for understanding tumorigenesis.
- Subcutaneous transplantation is common but lacks the native lung microenvironment.
- This limitation hinders the study of lung cancer development and progression.
Purpose of the Study:
- To develop a novel intrapulmonary (iPUL) orthotopic transplantation method for lung cancer research.
- To enable precise delivery of lung cancer cells to the mouse lung.
- To facilitate the study of lung cancer in its native microenvironment.
Main Methods:
- Development of an intrapulmonary (iPUL) orthotopic transplantation technique.
- Delivery of lung cancer cells to the left lung lobe of recipient mice.
- Integration of position emission tomography (PET) imaging for tumor detection.
Main Results:
- The iPUL method allows precise delivery and growth of lung cancer cells within the native lung microenvironment.
- Serial detection and tracking of tumor growth and dissemination over time were achieved using PET imaging.
- This model overcomes the limitations of subcutaneous transplantation for studying lung cancer.
Conclusions:
- The intrapulmonary (iPUL) orthotopic transplantation method is a valuable tool for lung cancer research.
- This technique, coupled with PET imaging, provides a more accurate preclinical model for studying lung tumorigenesis.
- It enables detailed investigation of lung tumor growth, dissemination, and response to therapies in a relevant microenvironment.
