Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Peptide Identification Using Tandem Mass Spectrometry01:33

Peptide Identification Using Tandem Mass Spectrometry

6.6K
Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...
6.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of demographic factors affecting predictability of the sacro-femoral-pubic angle in healthy adolescents.

Journal of anatomy·2014
Same author

Synergistic antitumor activity of withaferin A combined with oxaliplatin triggers reactive oxygen species-mediated inactivation of the PI3K/AKT pathway in human pancreatic cancer cells.

Cancer letters·2014
Same author

[Association between -1296T/C and -915A/G polymorphisms of matrix metalloproteinase inhibitor-3 gene and atherosclerotic cerebral infarction in an ethnic Han Chinese population].

Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics·2014
Same author

A preliminary study on surgery for hepatocellular carcinoma patients with portal hypertension.

American journal of surgery·2014
Same author

[Development of CBCT technique and its application on dental age assessment].

Fa yi xue za zhi·2014
Same author

[Functional hemodynamic monitoring should be emphasized in intensive care for burn and trauma patients].

Zhonghua shao shang za zhi = Zhonghua shaoshang zazhi = Chinese journal of burns·2014
Same journal

UPF3A and UPF3B shape the transcriptome cooperatively yet oppose cell function.

Journal of molecular biology·2026
Same journal

Antibody-secreting cells integrate efficient NMD with non‑canonical UPR signaling to maintain proteostasis and support massive immunoglobulin synthesis.

Journal of molecular biology·2026
Same journal

Small molecule stabilization of diverse amyloidogenic immunoglobulin light chains revealed by hydrogen-deuterium exchange mass spectrometry.

Journal of molecular biology·2026
Same journal

UPF1 at Work: Structural and Mechanistic Insights Into a Master Regulator of Nonsense-Mediated mRNA Decay.

Journal of molecular biology·2026
Same journal

Structural basis for the pro-amyloidogenic action and ligand binding of a novel W72R variant of human apolipoprotein A-I.

Journal of molecular biology·2026
Same journal

Cryo-EM Structure of the C. Elegans Septin Tetramer Reveals a Revised Architecture and Conserved Positional Orthology.

Journal of molecular biology·2026
See all related articles

Related Experiment Video

Updated: Jul 25, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.0K

SISPRO: Signature Identification for Spatial Proteomics.

Ying Zhou1, Yintao Zhang2, Fengcheng Li3

  • 1State Key Laboratory for Diagnosis and Treatment of Infectious Disease, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Zhejiang Provincial Key Laboratory for Drug Clinical Research and Evaluation, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310000, China. Electronic address: https://twitter.com/zhou36847275.

Journal of Molecular Biology
|June 25, 2023
PubMed
Summary
This summary is machine-generated.

Spatial proteomics identifies organelle proteins but lacks robustness and interpretation. The new SISPRO tool enhances proteomic signature accuracy and provides comprehensive spatial information for biological annotation.

Keywords:
accuracyorganelle-specific biological interpretationrobustnesssignature identificationspatial proteomics

More Related Videos

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF
05:52

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF

Published on: January 12, 2024

1.2K
A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation
14:44

A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

Published on: September 24, 2012

20.6K

Related Experiment Videos

Last Updated: Jul 25, 2025

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection
09:19

Spatial Profiling of Protein and RNA Expression in Tissue: An Approach to Fine-Tune Virtual Microdissection

Published on: July 6, 2022

5.0K
Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF
05:52

Author Spotlight: Enhanced Histone PTM Isomer Identification Through LC-TIMS-ToF MS/MS and PASEF

Published on: January 12, 2024

1.2K
A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation
14:44

A Protocol for the Identification of Protein-protein Interactions Based on 15N Metabolic Labeling, Immunoprecipitation, Quantitative Mass Spectrometry and Affinity Modulation

Published on: September 24, 2012

20.6K

Area of Science:

  • Proteomics
  • Cell Biology
  • Bioinformatics

Background:

  • Spatial proteomics is crucial for understanding cellular functions and disease mechanisms.
  • Current methods face limitations in the robustness of identified proteomic signatures and automated interpretation.
  • Lack of integrated subcellular information hinders the analysis of proteomic data.

Purpose of the Study:

  • To develop an online tool, SISPRO, for improved spatial proteomic signature identification and interpretation.
  • To enhance the robustness and accuracy of proteomic signature identification.
  • To integrate comprehensive subcellular information for automated biological annotation.

Main Methods:

  • Developed the SISPRO online tool.
  • Implemented a novel approach evaluating relative weighted consistency (CWrel) and area under the curve (AUC) for signature identification.
  • Integrated data from 9 organelles and 22 subcellular structures for annotation.

Main Results:

  • SISPRO successfully identifies proteomic signatures with improved robustness and accuracy.
  • The tool provides comprehensive spatial information, annotating proteins across multiple subcellular locations.
  • SISPRO facilitates automated interpretation of proteomic signatures by integrating diverse biological data.

Conclusions:

  • SISPRO offers a significant advancement in spatial proteomics by enhancing signature identification and biological annotation.
  • The tool addresses key limitations in robustness and interpretation, facilitating deeper insights into cellular processes.
  • SISPRO is a valuable, freely accessible resource for the spatial proteomics research community.