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Published on: May 27, 2016
[Expression and Clinical Significance of Exosome Derived MiR-181b-5p in Children with Acute Lymphoblastic Leukemia]
Yi Hong1, Kang-Kang Liu1, Ning-Ling Wang2
1Department of Pediatrics, The Second Hospital of Anhui Medical University, Hefei 230061, Anhui Province, China.
Insights
Exosomal miR-181b-5p levels are lower in newly diagnosed and relapsed acute lymphoblastic leukemia (ALL) in children. This microRNA can serve as a biomarker for monitoring ALL disease status.
Area of Science:
- Biochemistry
- Molecular Biology
- Pediatric Oncology
Context:
- Acute lymphoblastic leukemia (ALL) is a common childhood cancer.
- Exosomes play a role in intercellular communication and disease progression.
- MicroRNAs (miRNAs) are key regulators of gene expression.
Purpose:
- To investigate the expression levels of exosomal miR-181b-5p in children with ALL across different disease stages.
- To determine the relationship between exosomal miR-181b-5p levels and clinical characteristics in pediatric ALL.
Summary:
- Exosomal miR-181b-5p levels were significantly lower in newly diagnosed and relapsed ALL compared to remission and control groups.
- Higher exosomal miR-181b-5p expression was observed in T-ALL versus B-ALL and in male versus female children.
- These findings suggest dynamic changes in exosomal miR-181b-5p during ALL progression.
Impact:
- Exosomal miR-181b-5p can potentially serve as a non-invasive biomarker for monitoring ALL disease status in children.
- Exosomes represent a promising platform for targeted therapy delivery in ALL.
- Understanding miRNA dynamics in ALL offers insights into disease mechanisms and therapeutic strategies.
Objective:
To explore the expression level of exosome derived miR-181b-5p in different disease stages of children with acute lymphoblastic leukemia and its relationship with clinical characteristics.
Methods:
Bone marrow plasma samples of 86 children with ALL were collected. Exosomes were extracted by exosome extraction kit, and RNA in exosomes was extracted by TRIzol method. The levels of miR-181b-5p in the blood plasma exosomes of the patients in the newly diagnosed group, relapse group, remission group and control group were detected by qRT- PCR. The difference of miR-181b-5p expression level in each group was compared and analyzed, and the relationship between miR-181b-5p expression level and clinical characteristics was analyzed.
Results:
The expression level of exosomal miR-181b-5p in the newly diagnosed group and the relapsed group was significantly lower than that in the remission group and the control group (P< 0.05). The expression level of exosomal miR-181b-5p in T-ALL children was higher than that in B-ALL children (P<0.05). The expression level of plasma exosomal miR-181b-5p in male children was higher than that in female children (P<0.01).
Conclusion:
Exosome derived miR-181b-5p changes dynamically in the course of ALL children, and can be used as a marker miRNA to monitor disease status. Exosomes can transmit information in the tumor microenvironment and serve as a potential carrier for biomolecular targeted therapy.
