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Atomic-precise Pt2Cu4 cluster-based fluorescent sensor for rapid interleukin-6 detection
Zi-Hui Shao1, Hui-Lin Mo1, Xueli Zhao1
1Henan Key Laboratory of Crystalline Molecular Functional Materials, Henan International Joint Laboratory of Tumor Theranostic Cluster Materials, Green Catalysis Center and College of Chemistry, Zhengzhou University, Zhengzhou 450001, China. xuelizhao@zzu.edu.cn.
Analytical Methods : Advancing Methods and Applications
|June 26, 2023
Summary
Researchers developed fluorescent nanobeads using levonorgestrel-protected platinum-copper clusters. These nanobeads enable rapid and sensitive detection of interleukin-6 (IL-6), a key marker in inflammatory diseases.
Area of Science:
- Nanotechnology
- Biomedical Engineering
- Analytical Chemistry
Background:
- Interleukin-6 (IL-6) is a critical cytokine involved in inflammatory diseases.
- Sensitive detection of IL-6 is crucial for early diagnosis and monitoring of inflammatory conditions.
- Existing detection methods may lack the required speed, sensitivity, or simplicity.
Purpose of the Study:
- To develop a novel fluorescent nanobead (NB) platform for detecting interleukin-6 (IL-6).
- To establish an immunofluorescence sensor for rapid and sensitive IL-6 quantification.
- To evaluate the sensor's performance in detecting IL-6 in biological samples like serum.
Main Methods:
- Synthesis of levonorgestrel-protected platinum-copper (Pt2Cu4) clusters.
- Assembly of Pt2Cu4 clusters with a polymer to form intensely red-fluorescent nanobeads.
- Development of an immunofluorescence assay utilizing the Pt2Cu4 NBs for IL-6 detection.
Main Results:
- Successfully prepared Pt2Cu4 nanobeads exhibiting strong red fluorescence.
- Established an immunofluorescence sensor with a low limit of detection for IL-6 (42.66 pg mL-1).
- Demonstrated accurate detection of spiked IL-6 levels in human serum samples.
Conclusions:
- The developed Pt2Cu4 nanobead-based immunofluorescence sensor offers a promising tool for rapid and sensitive IL-6 detection.
- This platform has potential applications in diagnosing and monitoring inflammatory diseases.
- The study highlights the utility of functionalized metal clusters in developing advanced biosensing technologies.
