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A Procedure for Mouse Dorsal Root Ganglion Cryosectioning
Liangliang He1, Wenxing Zhao2, Lingyi Zhang3
1Department of Pain Management, Xuanwu Hospital, Capital Medical University; Department of Anesthesia and Perioperative Care, University of California San Francisco.
Journal of Visualized Experiments : Jove
|June 26, 2023
Summary
Obtaining high-quality dorsal root ganglion (DRG) cryostat sections for pain and neurological research is challenging. This protocol provides a step-by-step method to consistently achieve intact, flat DRG sections on slides.
Area of Science:
- Neuroscience
- Histology
- Pain Research
Background:
- High-quality mouse dorsal root ganglion (DRG) cryostat sections are essential for immunochemistry and RNAscope studies.
- Challenges exist in obtaining intact, flat sections from small DRG samples.
Purpose of the Study:
- To present an optimal, step-by-step protocol for cryosectioning mouse DRG tissue.
- To address common difficulties in DRG cryosectioning for improved research.
Main Methods:
- A detailed protocol for preparing and sectioning DRG tissue.
- Techniques for removing excess liquid, orienting sections, and flattening them on slides.
Main Results:
- Successfully developed a method to consistently obtain high-quality, intact, and flat DRG cryostat sections.
- The protocol effectively resolves common issues encountered during DRG cryosectioning.
Conclusions:
- This protocol offers a reliable solution for cryosectioning small DRG samples.
- The method is adaptable for cryosectioning other small tissue types.