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Prediction of Effector Proteins from Trichoderma longibrachiatum Through Transcriptome Sequencing.

Jialin Zhang¹, Lijun Wang¹, Aizhi Ren¹

¹Liaocheng University, Liaocheng, 252000, China.

Current Microbiology

|June 26, 2023

Summary

Trichoderma longibrachiatum SMF2 secretes effector proteins that may enhance plant growth and immunity. These findings reveal potential mechanisms for this biocontrol strain

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Area of Science:

Microbiology
Plant Pathology
Biotechnology

Background:

Trichoderma longibrachiatum SMF2 is a known biocontrol agent.
Understanding its mechanism is crucial for agricultural applications.

Purpose of the Study:

To identify and analyze effector proteins secreted by T. longibrachiatum SMF2.
To elucidate the role of these proteins in biocontrol and plant interaction.

Main Methods:

Bioinformatics analysis of secreted proteins.
Transcriptome sequencing to identify upregulated proteins.
Quantitative PCR validation of specific effector candidates.

Main Results:

Identified 478 secretory proteins from T. longibrachiatum.
Found 272 proteins upregulated after plant treatment.
36 secretory proteins showed homology to known effectors from pathogens.

Conclusions:

Secretory proteins from T. longibrachiatum SMF2 likely function as effectors.
These effectors may promote fungal growth, colonization, or induce plant immunity.