Jove
Visualize
Contact Us

Related Concept Videos

Photoluminescence: Applications01:14

Photoluminescence: Applications

440
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
440
Labeling DNA Probes03:31

Labeling DNA Probes

8.2K
DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
8.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Draft genome sequence of naphthalene-degrading marine <i>Rhodococcus ruber</i> PCTR3.

Microbiology resource announcements·2026
Same author

Integrative frameworks for plastic biodegradation in insect-microbiome systems: mechanistic insights, emerging multi-omics and enzyme engineering perspectives.

Biodegradation·2026
Same author

An imidazole-based fluorescent probe for the selective detection of copper (I) and copper (II) ions and potential applications.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2026
Same author

In silico analysis, expression, and characterization of LysStp1 endolysin with L-alanyl-D-glutamate carboxypeptidase activity.

Virus genes·2026
Same author

Characterization, genomic insights and anti-biofilm potential of phage vB_PaeM_PKMS3, a lytic Pbunavirus capable of infecting clinical Pseudomonas aeruginosa isolates.

Antonie van Leeuwenhoek·2026
Same author

Two-Step Non-Food Valorization of <i>Phaleria macrocarpa</i> Fruit Lignin into Lignin Nanoparticles and Quantum Dots for Antibacterial and Bioimaging Applications.

International journal of molecular sciences·2026
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jul 25, 2025

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
05:51

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

1.9K

A Porphyrin-based NIR Fluorescent Probe for Bi3+ and Potential Applications.

Pranati Somkuwar1, R Bhaskar1, Selva Kumar Ramasamy2

  • 1Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Vellore-632014, Tamil Nadu, India.

Journal of Fluorescence
|June 26, 2023
PubMed
Summary

A novel porphyrin probe (P) selectively detects bismuth ions (Bi3+) with high sensitivity, enabling colorimetric and fluorogenic analysis in water samples. This probe offers a new method for bismuth detection.

Keywords:
BismuthColorimetryDFT, BioimagingFluorometryPorphyrinQuenching

More Related Videos

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
12:52

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

Published on: July 7, 2015

9.2K
Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
10:38

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Published on: March 3, 2010

14.0K

Related Experiment Videos

Last Updated: Jul 25, 2025

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
05:51

A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging

Published on: March 17, 2023

1.9K
IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II
12:52

IridiumIII Luminescent Probe for Detection of the Malarial Protein Biomarker Histidine Rich Protein-II

Published on: July 7, 2015

9.2K
Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems
10:38

Synthesis and Calibration of Phosphorescent Nanoprobes for Oxygen Imaging in Biological Systems

Published on: March 3, 2010

14.0K

Area of Science:

  • Analytical Chemistry
  • Materials Science
  • Biochemistry

Background:

  • Selective and sensitive detection of metal ions is crucial in environmental monitoring and biological studies.
  • Bismuth ions (Bi3+) play roles in various industrial applications and biological processes, necessitating reliable detection methods.
  • Existing methods for Bi3+ detection often lack selectivity or sensitivity, driving the need for novel probes.

Purpose of the Study:

  • To develop and characterize a new porphyrin-based probe (P) for the selective and sensitive detection of Bi3+ ions.
  • To investigate the photophysical properties and binding mechanism of probe P with Bi3+.
  • To evaluate the applicability of probe P for quantitative Bi3+ determination in real-world samples and assess its biocompatibility.

Main Methods:

  • Synthesis and characterization of 5,10,15,20-Tetrakis(4-hydroxyphenyl) porphyrin (P) using NMR, IR, and ESI-MS.
  • Spectrophotometric and spectrofluorometric studies in DMSO:H2O (8:2, v/v) media to assess photophysical properties and selectivity.
  • Job's plot analysis, Stern-Volmer quenching studies, and DFT calculations to elucidate binding stoichiometry and mechanism.

Main Results:

  • Probe P exhibited selective red fluorescence quenching in the presence of Bi3+ ions, with no significant response to other metal ions.
  • A 1:1 stoichiometric binding ratio between probe P and Bi3+ was determined, with an association constant of 3.4 ×105 M-1.
  • The probe achieved a detection limit of 27 nM for Bi3+ and demonstrated successful quantitative determination in water samples with good biocompatibility.

Conclusions:

  • The synthesized porphyrin probe P is highly selective and sensitive for Bi3+ detection in semi-aqueous media.
  • Probe P represents the first reported colorimetric and fluorogenic probe for Bi3+ detection.
  • The probe's promising performance and biocompatibility suggest its potential for practical applications in environmental and biological monitoring.