Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Synthesis and Regulation of Thyroid Hormones01:20

Synthesis and Regulation of Thyroid Hormones

4.8K
Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The...
4.8K
Atomic Absorption Spectroscopy: Interference01:25

Atomic Absorption Spectroscopy: Interference

856
Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
856
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

529
Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and...
529
Atomic Emission Spectroscopy: Interference01:30

Atomic Emission Spectroscopy: Interference

233
In atomic emission spectroscopy (AES), high-temperature atomizers excite a broad range of elements and molecules that generate complex emissions from sources such as oxides, hydroxides, and flame combustion products in the flame or plasma. Several strategies can be employed to minimize spectral interferences caused by overlapping emission lines or bands. These include increasing instrument resolution, choosing alternative emission lines, optimally placing the detector in low-background regions,...
233
Errors occurring during blood pressure monitoring01:25

Errors occurring during blood pressure monitoring

781
Blood pressure monitoring is a crucial clinical procedure in diagnosing and managing various cardiovascular conditions. Despite its significance, the accuracy of blood pressure measurements can be compromised by multiple factors, potentially leading to either falsely high or low readings. These inaccuracies are critical as they can significantly impact patient care. So, it is vital to understand these challenges deeply and adopt strategic approaches to minimize errors.
Several factors...
781

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A New Model for Screening for Late-Onset Preeclampsia in the Third Trimester.

Journal of clinical medicine·2025
Same author

Screening and diagnosis of primary aldosteronism. Consensus document of all the Spanish Societies involved in the management of primary aldosteronism.

Endocrine·2024
Same author

Relevance of inferior petrosal sinus sampling in the diagnosis of Cushing's syndrome: a case report.

Advances in laboratory medicine·2023
Same author

Macroprolactin: From laboratory to clinical practice.

Endocrinologia, diabetes y nutricion·2022
Same author

Macroprolactin: From laboratory to clinical practice.

Endocrinologia, diabetes y nutricion·2021
Same author

Validation of the human chorionic gonadotropin immunoassay in cerebrospinal fluid for the diagnostic work-up of neurohypophyseal germinomas.

Annals of clinical biochemistry·2011

Related Experiment Video

Updated: Jul 25, 2025

Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species
07:37

Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species

Published on: February 21, 2020

7.0K

False hyperthyroidism caused by interference in immunoassays.

Clara Jiménez García1, Piedad Ortega Fernández2, María Eugenia Torregrosa Quesada1

  • 1Laboratory of Hormones, Hospital General Universitario de Alicante, Pintor Baeza 11, Alicante, Spain.

Advances in Laboratory Medicine
|June 26, 2023
PubMed
Summary

Thyroid function immunoassays can be affected by interference, leading to inaccurate results. This case highlights how interfering agents impacted thyroid-stimulating hormone (TSH) and free thyroxine (FT4) measurements in a patient, despite her lack of symptoms.

Keywords:
heterophilic antibodieshyperthyroidisminterference of immunoassay

More Related Videos

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model
04:39

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

Published on: March 17, 2023

1.7K
A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases
06:50

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases

Published on: May 29, 2020

2.6K

Related Experiment Videos

Last Updated: Jul 25, 2025

Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species
07:37

Microwaving and Fluorophore-Tyramide for Multiplex Immunostaining on Mouse Adrenals − Using Unconjugated Primary Antibodies from the Same Host Species

Published on: February 21, 2020

7.0K
Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model
04:39

Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model

Published on: March 17, 2023

1.7K
A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases
06:50

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases

Published on: May 29, 2020

2.6K

Area of Science:

  • Clinical Chemistry
  • Endocrinology
  • Laboratory Medicine

Background:

  • Immunoassays for thyroid function testing are susceptible to various interferences.
  • Interference can lead to misinterpretation of results and impact clinical decisions.
  • Accurate thyroid hormone assessment is crucial for patient management.

Purpose of the Study:

  • To report a case of methodological interference affecting thyroid function tests.
  • To investigate the cause of discrepancy between clinical presentation and laboratory findings.
  • To identify interfering agents impacting thyrotropin (TSH) and free thyroxine (FT4) assays.

Main Methods:

  • Case presentation of a patient with iatrogenic hypothyroidism post-radioiodine therapy.
  • Clinical evaluation and biochemical assessment of thyroid function.
  • Laboratory investigation to identify and exclude methodological interference in TSH and FT4 assays.

Main Results:

  • A 37-year-old asymptomatic woman presented with biochemical findings of hypothyroidism (TSH: 7.75 mU/L, FT4: >7.7 ng/dL).
  • Discrepancy between clinical status and lab results suggested interference.
  • Interfering agents were identified, affecting both TSH and FT4 measurements.

Conclusions:

  • Methodological interference can significantly impact thyroid function test results.
  • Thorough laboratory evaluation is essential to detect interference when clinical and biochemical data are discordant.
  • Identification of interfering agents is critical for accurate thyroid hormone determination.